Expression patterns of chitinase and chitosanase produced from Bacillus cereus in suppression of phytopathogen

被引：10

作者：

Se, Dong-Jun ^{[1
]}

Lee, Ji-Hae ^{[1
]}

Song, Yong-Su ^{[1
]}

Park, Ro-Dong ^{[1
]}

Jung, Woo-Jin ^{[1
]}

机构：

[1] Chonnam Natl Univ, Coll Agr & Life Sci, Inst Environm Friendly Agr, Div Appl Biosci & Biotechnol, Kwangju 500757, South Korea

来源：

MICROBIAL PATHOGENESIS | 2014年 / 73卷

关键词：

Chitinase activity; Chitosanase activity; Isozyme; Bacillus cereus; PURIFICATION; STRAIN; OLIGOSACCHARIDES; CHITIN/CHITOSAN; CLONING; ENZYME;

D O I：

10.1016/j.micpath.2014.05.007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Bacillus cereus MP-310 was incubated on various culture media substrates as LB, colloidal chitin, chitosan powder, and chitosan beads to investigate the concurrent expression patterns of chitinase and chitosanase isozymes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Chitinase activity increased rapidly with a maximum level after 6 days of incubation in CM-chitin medium. Major bands of chitinase isozymes were strongly expressed on SDS-PAGE in LB medium (four bands) and in colloidal chitin medium (five bands) after 6 days after incubation, and in chitosan powder medium (one band) and in chitosan beads medium (five bands) after 12 days after incubation. A major band of chitosanase isozymes was strongly expressed on SDS-PAGE in chitosan powder medium (one band) and in chitosan beads medium (one band) after 12 days of incubation. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：31 / 36

页数：6

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