Colorectal cancer patients with different C reactive protein levels and 5-year survival times can be differentiated with quantitative serum proteomics
被引:32
作者:
Holm, Matilda
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Univ Helsinki, Dept Pathol, Helsinki, Finland
Helsinki Univ Hosp, Helsinki, Finland
Univ Helsinki, Dept Surg, Helsinki, Finland
Univ Helsinki, Res Programs Unit, Genome Scale Biol Res Program, Helsinki, Finland
Univ Helsinki, Translat Canc Biol Res Program, Res Programs Unit, Helsinki, FinlandUniv Helsinki, Dept Pathol, Helsinki, Finland
Holm, Matilda
[1
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Saraswat, Mayank
[6
,7
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Joenvaara, Sakari
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机构:
Univ Helsinki, Haartman Inst, Transplantat Lab, Helsinki, Finland
Helsinki Univ Hosp, HUSLAB, Helsinki, FinlandUniv Helsinki, Dept Pathol, Helsinki, Finland
Joenvaara, Sakari
[6
,7
]
Ristimaki, Ari
论文数: 0引用数: 0
h-index: 0
机构:
Univ Helsinki, Dept Pathol, Helsinki, Finland
Helsinki Univ Hosp, Helsinki, Finland
Univ Helsinki, Res Programs Unit, Genome Scale Biol Res Program, Helsinki, Finland
Helsinki Univ Hosp, HUSLAB, Helsinki, FinlandUniv Helsinki, Dept Pathol, Helsinki, Finland
Ristimaki, Ari
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Haglund, Caj
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Renkonen, Risto
[6
,7
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机构:
[1] Univ Helsinki, Dept Pathol, Helsinki, Finland
[2] Helsinki Univ Hosp, Helsinki, Finland
[3] Univ Helsinki, Dept Surg, Helsinki, Finland
[4] Univ Helsinki, Res Programs Unit, Genome Scale Biol Res Program, Helsinki, Finland
[5] Univ Helsinki, Translat Canc Biol Res Program, Res Programs Unit, Helsinki, Finland
[6] Univ Helsinki, Haartman Inst, Transplantat Lab, Helsinki, Finland
Over 1.4 million people are diagnosed with colorectal cancer (CRC) each year, making it the third most common cancer in the world. Increased screening and therapeutic modalities including improved combination treatments have reduced CRC mortality, although incidence and mortality rates are still increasing in some areas. Serum-based biomarkers are mainly used for follow-up of cancer, and are ideal due to the ease and minimally invasive nature of sample collection. Unfortunately, CEA and other serum markers have too low sensitivity for screening and preoperative diagnostic purposes. Increasing interest is focused on the possible use of biomarkers for predicting treatment response and prognosis in cancer. In this study, we have performed mass spectrometry analysis (UPLC-UDMSE) of serum samples from 19 CRC patients. Increased levels of C-reactive protein (CRP), which occur during local inflammation and the presence of a systemic inflammatory response, have been linked to poor prognosis in CRC patients. We chose to analyze samples according to CRP values by dividing them into the categories CRP <30 and >30, and, separately, according to short and long 5-year survival. The aim was to discover differentially expressed proteins associated with poor prognosis and shorter survival. We quantified 256 proteins and performed detailed statistical analyses and pathway analysis. We discovered multiple proteins that are up- or downregulated in patients with CRP >30 as compared to CRP <30 and in patients with short as compared to long 5-year survival. Pathways that were enriched include LXR/RXR activation, FXR/RXR activation, complement and coagulation cascades and acute phase signaling response, with some of the proteins we identified having roles in these pathways. In this study, we have identified multiple proteins, of which a few have been previously identified as potential biomarkers, and others that have been identified as potential biomarkers for CRC for the first time, to the best of our knowledge. While these proteins still need to be validated in larger patient series, this pilot study will pave the way for future studies aiming to provide better biomarkers for patients with CRC.
机构:
Univ Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Blakesley, Richard E.
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Mazumdar, Sati
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机构:
Univ Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Mazumdar, Sati
;
Dew, Mary Amanda
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机构:
Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15260 USA
Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15260 USA
Univ Pittsburgh, Dept Psychol, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Dew, Mary Amanda
;
Houck, Patricia R.
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机构:
Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Houck, Patricia R.
;
Tang, Gong
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机构:
Univ Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Tang, Gong
;
Reynolds, Charles F., III
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机构:
Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Reynolds, Charles F., III
;
Butters, Meryl A.
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机构:
Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
机构:
Queen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R ChinaQueen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R China
Cho, W. C. S.
;
Yip, T. T.
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机构:
Ciphergen Biosyst Inc, Fremont, CA USAQueen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R China
Yip, T. T.
;
Cheng, W. W.
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机构:
Queen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R ChinaQueen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R China
Cheng, W. W.
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Au, J. S. K.
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机构:
Queen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R ChinaQueen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R China
机构:
Natl Hlth Res Inst, Inst Cellular & Syst Med, Zhunan Town 350, Miaoli County, Taiwan
Natl Hlth Res Inst, Translat Ctr Glandular Malignancies, Zhunan Town 350, Miaoli County, TaiwanNatl Hlth Res Inst, Inst Cellular & Syst Med, Zhunan Town 350, Miaoli County, Taiwan
机构:
Univ Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Blakesley, Richard E.
;
Mazumdar, Sati
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Mazumdar, Sati
;
Dew, Mary Amanda
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15260 USA
Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15260 USA
Univ Pittsburgh, Dept Psychol, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Dew, Mary Amanda
;
Houck, Patricia R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Houck, Patricia R.
;
Tang, Gong
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Tang, Gong
;
Reynolds, Charles F., III
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
Reynolds, Charles F., III
;
Butters, Meryl A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15260 USAUniv Pittsburgh, Dept Biostat, Pittsburgh, PA 15260 USA
机构:
Queen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R ChinaQueen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R China
Cho, W. C. S.
;
Yip, T. T.
论文数: 0引用数: 0
h-index: 0
机构:
Ciphergen Biosyst Inc, Fremont, CA USAQueen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R China
Yip, T. T.
;
Cheng, W. W.
论文数: 0引用数: 0
h-index: 0
机构:
Queen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R ChinaQueen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R China
Cheng, W. W.
;
Au, J. S. K.
论文数: 0引用数: 0
h-index: 0
机构:
Queen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R ChinaQueen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R China
机构:
Natl Hlth Res Inst, Inst Cellular & Syst Med, Zhunan Town 350, Miaoli County, Taiwan
Natl Hlth Res Inst, Translat Ctr Glandular Malignancies, Zhunan Town 350, Miaoli County, TaiwanNatl Hlth Res Inst, Inst Cellular & Syst Med, Zhunan Town 350, Miaoli County, Taiwan