Effect of osmolytes on the fibrillation of HypF-N

被引：4

作者：

Bhavsar, Rupen D. ^{[1
]}

Prasad, Shivcharan ^{[1
]}

Roy, Ipsita ^{[1
]}

机构：

[1] NIPER, Dept Biotechnol, Sas Nagar 160062, Punjab, India

来源：

BIOCHIMIE | 2013年 / 95卷 / 11期

关键词：

Fibrillation; HypF-N; Osmolytes; Polyols; Proline; PROTEIN STABILITY; L-PROLINE; WATER; AGGREGATION; HYDRATION;

D O I：

10.1016/j.biochi.2013.07.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the effect of a series of stabilizing and destabilizing osmolytes on the fibrillation pattern of a model amyloidogenic protein, HypF-N. Under mildly denaturing conditions, HypF-N forms cross beta-sheet structures, characteristic of amyloid fibrils. In the presence of all stabilizing osmolytes except proline, fibrillation of HypF-N is inhibited. Notably, fibrillation kinetics is retarded at subdenaturing concentrations of chaotropes. In case of proline, fibrillation of HypF-N is accelerated. Thus, the changes during exposure of a protein to denaturing conditions in the presence of osmolyes cannot be extrapolated from their role as anti-fibrillation agents. (C) 2013 Elsevier Masson SAS. All rights reserved.

引用

页码：2190 / 2193

页数：4

共 50 条

[1] Conformational Properties of Unfolded HypF-N
Chen, Yujie
Parrini, Claudia
Taddei, Niccolo
Lapidus, Lisa J.
JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (50): : 16209 - 16213
[2] Conformational properties of the aggregation precursor state of HypF-N
Campioni, Silvia
Mossuto, Maria F.
Torrassa, Silvia
Calloni, Giulia
de Laureto, Patrizia Polverino
Relini, Annalisa
Fontana, Angelo
Chiti, Fabrizio
JOURNAL OF MOLECULAR BIOLOGY, 2008, 379 (03) : 554 - 567
[3] Glycosaminoglycans (GAGs) Suppress the Toxicity of HypF-N Prefibrillar Aggregates
Saridaki, Theodora
Zampagni, Mariagioia
Mannini, Benedetta
Evangelisti, Elisa
Taddei, Niccolo
Cecchi, Cristina
Chiti, Fabrizio
JOURNAL OF MOLECULAR BIOLOGY, 2012, 421 (4-5) : 616 - 630
[4] Proteomic analysis of cells exposed to prefibrillar aggregates of HypF-N
Magherini, Francesca
Pieri, Laura
Guidi, Francesca
Giangrande, Chiara
Amoresano, Angela
Bucciantini, Monica
Stefani, Massimo
Modesti, Alessandra
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2009, 1794 (08): : 1243 - 1250
[5] Structural differences between toxic and nontoxic HypF-N oligomers
Capitini, Claudia
Patel, Jayneil R.
Natalello, Antonino
D'Andrea, Cristiano
Relini, Annalisa
Jarvis, James A.
Birolo, Leila
Peduzzo, Alessia
Vendruscolo, Michele
Matteini, Paolo
Dobson, Christopher M.
De Simone, Alfonso
Chiti, Fabrizio
CHEMICAL COMMUNICATIONS, 2018, 54 (62) : 8637 - 8640
[6] Toxic oligomers of the amyloidogenic HypF-N protein form pores in mitochondrial membranes
Maria Ylenia Farrugia
Mario Caruana
Stephanie Ghio
Angelique Camilleri
Claude Farrugia
Ruben J. Cauchi
Sara Cappelli
Fabrizio Chiti
Neville Vassallo
Scientific Reports, 10
[7] Toxic oligomers of the amyloidogenic HypF-N protein form pores in mitochondrial membranes
Farrugia, Maria Ylenia
Caruana, Mario
Ghio, Stephanie
Camilleri, Angelique
Farrugia, Claude
Cauchi, Ruben J.
Cappelli, Sara
Chiti, Fabrizio
Vassallo, Neville
SCIENTIFIC REPORTS, 2020, 10 (01)
[8] Amyloid-β oligomer synaptotoxicity is mimicked by oligomers of the model protein HypF-N
Tatini, Francesca
Pugliese, Anna Maria
Traini, Chiara
Niccoli, Sandra
Maraula, Giovanna
Dami, Teresa Ed
Mannini, Benedetta
Scartabelli, Tania
Pedata, Felicita
Casamenti, Fiorella
Chiti, Fabrizio
NEUROBIOLOGY OF AGING, 2013, 34 (09) : 2100 - 2109
[9] Studying the HypF-N fibrillization path and precursors-induced permeability changes in liposoes
Gliozzi, A
Relini, A
Canale, C
Rolandi, R
Bucciantini, M
Stefani, M
BIOPHYSICAL JOURNAL, 2004, 86 (01) : 507A - 508A
[10] Toxic HypF-N Oligomers Selectively Bind the Plasma Membrane to Impair Cell Adhesion Capability
Oropesa-Nunez, Reinier
Keshavan, Sandeep
Dante, Silvia
Diaspro, Alberto
Mannini, Benedetta
Capitini, Claudia
Cecchi, Cristina
Stefani, Massimo
Chiti, Fabrizio
Canale, Claudio
BIOPHYSICAL JOURNAL, 2018, 114 (06) : 1357 - 1367

← 1 2 3 4 5 →