Production of 3-Hydroxypropionaldehyde in Silage Inoculated with Lactobacillus coryniformis Plus Glycerol

被引:19
作者
Tanaka, Osamu [1 ]
Komatsu, Tokushi [1 ]
Oshibe, Akinori [1 ]
Cai, Yimin [2 ]
Miyazaki, Shigeru [3 ]
Nakanishi, Kotoyoshi [4 ]
机构
[1] Natl Agr Res Ctr Tohoku Reg, Morioka, Iwate 0200198, Japan
[2] Natl Inst Livestock & Grassland Sci, Nasushiobara, Tochigi 3292793, Japan
[3] Natl Inst Anim Hlth, Tsukuba, Ibaraki 3050856, Japan
[4] Tokyo Univ Agr, Dept Brewing & Fermentat, Setagaya Ku, Tokyo 1568502, Japan
关键词
colorimetric method; 3-hydroxypropionaldehyde; Lactobacillus coryniformis; silage; LACTIC-ACID BACTERIA; AEROBIC STABILITY; FERMENTATION;
D O I
10.1271/bbb.80863
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We examined the production of an antimicrobial component, 3-hydroxypropionaldehyde (3-HPA), in laboratory-scale silage inoculated with Lactobacillus coryniformis strain 394; which ferments glycerol to 3-HPA. A modified colorimetric method that used an NaOH-treated blank and determined the absorption spectrum of the samples was employed to detect a 3-HPA-like component (HLC) that was assumed to be 3-HPA. Inoculation with Lb. coryniformis 394 plus glycerol in ensiling produced HLC at 10-460 ppm and contributed to inhibition of butyric fermentation and retardation of aerobic spoilage. HLC was considered to be 3-HPA from its absorption spectrum. These results suggest that the production of 3-HPA by Lb. coryniformis 394 is useful in ensiling and that the modified colorimetric method is effective to detect 3-HPA in silage.
引用
收藏
页码:1494 / 1499
页数:6
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