Microsatellite DNA markers for population genetic studies in the dinoflagellate Karenia brevis

被引:8
|
作者
Renshaw, Mark A. [1 ]
Soltysiak, Kelly
Arreola, Daniel
Loret, Pascal
Patton, John C.
Gold, John R.
Campbell, Lisa
机构
[1] Texas A&M Univ, Ctr Biosystemat & Biodivers, Dept Wildlife & Fisheries Sci, Coll Stn, College Stn, TX 77843 USA
[2] Texas A&M Univ, Dept Oceanog, College Stn, TX 77843 USA
来源
MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 04期
关键词
genomic library; harmful algal species; Karenia brevis; microsatellites;
D O I
10.1111/j.1471-8286.2006.01471.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nine nuclear-encoded microsatellites from an enriched genomic DNA library of the HAB (harmful algal bloom) dinoflagellate Karenia brevis were isolated and characterized. The microsatellites include five perfect (three dinucleotide and two trinucleotide) and four imperfect (two dinucleotide and two trinucleotide) repeat motifs. Gene (haplotype) diversity ranged from 0.153 to 0.750 among a sample of 13 isolates; the number of alleles among the isolates ranged from two to six and pairwise tests of genotypic disequilibria were nonsignificant. The microsatellites developed in this study will provide insight into the genetic diversity of this HAB species and tools that may prove useful in predicting source populations and physiological parameters of individual K. brevis blooms.
引用
收藏
页码:1157 / 1159
页数:3
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