Expression changes of BIK in breast cancer tissues of different histological subtypes

被引:2
作者
Ruiz-Esparza-Garrido, Ruth [1 ]
Torres-Lopez, Javier [2 ]
Pina-Sanchez, Patricia [3 ]
Viedma-Rodriguez, Rubi [4 ]
Rivera-Gonzalez, Alejandro [1 ]
Velazquez-Flores, Miguel A. [1 ]
机构
[1] Inst Mexicano Seguro Social, Hosp Pediat Silvestre Frenk Freund, Ctr Med Nacl Siglo XXI, Lab Genom Func,Unidad Invest Med Genet Humana, Ciudad De Mexico, Mexico
[2] Inst Mexicano Seguro Social, Ctr Med Nacl Siglo XXI, Unidad Invest Med Enfermedades Infecciosas, Unidades Med Alta Especialidad Pediat, Ciudad De Mexico, Mexico
[3] Inst Mexicano Seguro Social, Hosp Oncol, Ctr Med Nacl Siglo XXI, Unidad Invest Med Enfermedades Oncol, Ciudad De Mexico, Mexico
[4] Univ Autonoma Mexico, Fac Estudios Super Iztacala, Lab Biol Desarollo, Unidad Morfol & Func Celular, Ciudad De Mexico, Estado De Mexic, Mexico
来源
CIRUGIA Y CIRUJANOS | 2020年 / 88卷 / 02期
关键词
BIK; Breast cancer; Tissue microarray; Tumor suppressor; Oncogene; ENDOPLASMIC-RETICULUM; BH3-ONLY PROTEINS; GENE-EXPRESSION; CELL; INVOLVEMENT; RELEASE; NBK/BIK; DEATH; BAX;
D O I
10.24875/CIRU.19001039
中图分类号
R61 [外科手术学];
学科分类号
摘要
Objective: The objective of the study was to determine the expression levels of BIK in breast cancer (BC) tissues of different histological subtype and to delve into the participation of BIK in this type of cancer. Materials and methods: B/K and p-BIK (the phosphorylated form) protein expressions were tested by immunohistochemistry in BC tissue microarrays (Tumoral In = 90] and adjacent In = 40] tissues). Results: The data revealed an overexpression of B/K in invasive ductal (Grades I, IIA, and IIB) and in lobular (Grades HA and IIB) carcinomas compared to their respective adjacent tissues. By contrast, canalicular carcinoma (Grades I and 11B) and phyllodes tumors had very low expression levels of BIK. Only levels of p-BIK were shown to be increased in invasive ductal carcinoma (Grades I, IIA, and IIB). Meanwhile, quantitative polymerase chain reaction analysis showed lower B/K levels in MCF-10A and MCF-7 cells than in MDA-MB-231 and human mammary epithelial cells. In agreement with this, B/K protein was shown to be overexpressed in MDA-MB 231 relative to MCF-7 cells. Conclusions: Our results showed an association between BIK expression and the BC tumor subtype under study, which could be related to different OK functions in the BC subtypes.
引用
收藏
页码:163 / 169
页数:7
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