Cytosine-specific chemical probing of DNA using bromide and monoperoxysulfate

被引：51

作者：

Ross, SA ^{[1
]}

Burrows, CJ ^{[1
]}

机构：

[1] UNIV UTAH,DEPT CHEM,SALT LAKE CITY,UT 84112

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 24期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1093/nar/24.24.5062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bromination of cytosine and formation of a piperidine-labile site are observed when two simple salts, KBr and KHSO5, are allowed to react with single-stranded oligodeoxynucleotides. Selectivity for C compared with T, G or A is typically a factor of 4 or more; selectivity for Cs in a single-stranded region such as a C-bulge is nearly a factor of 10 compared with duplex Cs, Low reactivity and little base selectivity are observed using duplex DNA, although increased concentrations of reagents lead to complete degradation of the DNA, The results suggest that these conditions for in situ generation of Br-2 constitute a useful tool for examination of the exposure of a non-duplex cytosine base in folded DNA structures.

引用

页码：5062 / 5063

页数：2

共 12 条

[1]

AMBROSE BJB, 1987, METHOD ENZYMOL, V152, P522

[2] RECOGNITION OF GUANINE STRUCTURE IN NUCLEIC-ACIDS BY NICKEL-COMPLEXES [J].