PRELIMINARY STRUCTURAL CHARACTERIZATION, ANTIOXIDANT AND ANTITUMOR ACTIVITIES OF CHINESE CHESTNUT (CASTANEA MOLLISSIMA BLUME) POLYSACCHARIDES

被引：1

作者：

Wang, Tong-Kun ^{[1
]}

Du, Bin ^{[1
]}

Yang, Yue-Dong ^{[1
]}

Liang, Xue ^{[1
]}

Wang, Xiao-Hong ^{[1
]}

Zhou, Yan ^{[1
]}

机构：

[1] Hebei Normal Univ Sci & Technol, Hebei Chestnut Engn Technol Res Ctr, Qinhuangdao 066600, Hebei, Peoples R China

来源：

CURRENT TOPICS IN NUTRACEUTICAL RESEARCH | 2015年 / 13卷 / 04期

关键词：

Antioxidant activity; Antitumor activity; Castanea mollissima Blume; Characterization; Chinese chestnut; Isolation; Polysaccharide; MASS-SPECTROMETRY; PURIFICATION; CULTIVARS; VARIETIES; GLUCAN;

D O I：

暂无

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Anew polysaccharide was extracted from Chinese chestnut (Castanea mollissima Blume) by pressurized liquid extraction (PLE) and further successive purification through DEAF-cellulose 52 and Sephadex G-200 columns. The results showed a single and symmetrical sharp peak, which suggested that purified polysaccharide was homogeneous. The molecular weight was calculated to be 3.28 x 10(5) Da. Monosaccharide analysis revealed that the polysaccharide was composed of rhamnose, ribose, arabinose, xylose, mannose, glucose, and D-galactose in the molar ratio of 1.42%, 13.12%, 22.68%, 1.57%, 9.39%, 21.58%, 30.24%, respectively. There was a dose-dependent relationship with measures of antioxidant activities, viz. capabilities to scavenge free radical superoxide anion free radical, hydrogen peroxides and reducibility. The antitumor activity of the polysaccharide was tested in a cell culture model. The polysaccharide caused a significant dose dependent inhibition on the multiplication of cancer cells.

引用

页码：241 / 248

页数：8

共 30 条

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