Quantum Dot Triexciton Imaging with Three-Dimensional Subdiffraction Resolution

被引：33

作者：

Hennig, Simon ^{[1
]}

van de Linde, Sebastian ^{[1
]}

Heilemann, Mike ^{[1
]}

Sauer, Markus ^{[1
]}

机构：

[1] Univ Bielefeld, Bielefeld Inst Biophys & Nanosci, D-33615 Bielefeld, Germany

来源：

NANO LETTERS | 2009年 / 9卷 / 06期

关键词：

FIELD OPTICAL NANOSCOPY; FLUORESCENCE MICROSCOPY; CELLS; LIVE; NANOCRYSTALS; PROBES;

D O I：

10.1021/nl9012387

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe a simple method that improves optical resolution in fluorescence microscopy approximately 1.7-fold in all three dimensions and can be implemented on any basic confocal scanning microscope. This approach is based on three-photon absorption of commercially available quantum dots generating a triple exciton (triexciton) and subsequent blue-shifted fluorescence emission following recombination of the triexciton. As a pure physical approach, the resolution enhancement is independent from the nanoenvironment and demonstrated to work in living cells.

引用

收藏

页码：2466 / 2470

页数：5

相关论文

共 33 条

[1]

Abbe E., 1873, Arch. Mikrosk. Anat., V9, P413, DOI DOI 10.1007/BF02956173

[2] Semiconductor clusters, nanocrystals, and quantum dots [J].

Alivisatos, AP .

SCIENCE, 1996, 271 (5251) :933-937

[3] Spectral and dynamical characterization of multiexcitons in colloidal CdSe semiconductor quantum dots [J].

Bonati, C ;

Mohamed, MB ;

Tonti, D ;

Zgrablic, G ;

Haacke, S ;

van Mourik, F ;

Chergui, M .

PHYSICAL REVIEW B, 2005, 71 (20)

[4] Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species [J].

Bossi, Mariano ;

Foelling, Jonas ;

Belov, Vladimir N. ;

Boyarskiy, Vadim P. ;

Medda, Rebecca ;

Egner, Alexander ;

Eggeling, Christian ;

Schoenle, Andreas ;

Hell, Stefan W. .

NANO LETTERS, 2008, 8 (08) :2463-2468

[5] Fluorescent CdSe/ZnS nanocrystal-peptide conjugates for long-term, nontoxic imaging and nuclear targeting in living cells [J].

Chen, FQ ;

Gerion, D .

NANO LETTERS, 2004, 4 (10) :1827-1832

[6] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].

DENK, W ;

STRICKLER, JH ;

WEBB, WW .

SCIENCE, 1990, 248 (4951) :73-76

[7] Room-temperature ordered photon emission from multiexciton states in single CdSe core-shell nanocrystals [J].

Fisher, B ;

Caruge, JM ;

Zehnder, D ;

Bawendi, M .

PHYSICAL REVIEW LETTERS, 2005, 94 (08)

[8] Multiexciton fluorescence from semiconductor nanocrystals [J].

Fisher, B ;

Caruge, JM ;

Chan, YT ;

Halpert, J ;

Bawendi, MG .

CHEMICAL PHYSICS, 2005, 318 (1-2) :71-81

[9] Radiative recombination of triexcitons in CdSe colloidal quantum dots [J].

Franceschetti, A. ;

Troparevsky, M. C. .

JOURNAL OF PHYSICAL CHEMISTRY C, 2007, 111 (17) :6154-6157

[10] Multiplexed toxin analysis using four colors of quantum dot fluororeagents [J].

Goldman, ER ;

Clapp, AR ;

Anderson, GP ;

Uyeda, HT ;

Mauro, JM ;

Medintz, IL ;

Mattoussi, H .

ANALYTICAL CHEMISTRY, 2004, 76 (03) :684-688

← 1 2 3 4 →