Fluorescence turn-on assay for detection of serum D-penicillamine based on papain@AuNCs-Cu2+ complex

被引：40

作者：

Chen, Yifan ^{[1
,3
]}

Qiao, Juan ^{[1
,2
]}

Liu, Qianrong ^{[1
,4
]}

Zhang, Mingming ^{[1
,5
]}

Qi, Li ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, 2 Zhongguancun Beiyijie, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China

[3] Jilin Univ, Zhuhai Coll, Zhuhai 519041, Peoples R China

[4] Xian Univ Sci & Technol, Xian 710054, Shaanxi, Peoples R China

[5] Tianjin Normal Univ, Tianjin 300387, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2018年 / 1026卷

基金：

中国国家自然科学基金;

关键词：

Papain@AuNCs; Fluorescence turn on; D-penicillamine; Metabolic process; GOLD NANOCLUSTERS; SWITCH SENSOR; CARBON DOTS; PROBE; IONS; NANOPARTICLES; CU2+; GENERATION; SAMPLES; SYSTEM;

D O I：

10.1016/j.aca.2018.04.014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Papain-stabilized gold nanoclusters (papain@AuNCs) with a red fluorescence emission at 639 nm have been generated successfully in aqueous solution. The fluorescence of papain@AuNCs could be quenched in the addition of Cu2+. Subsequently, a unique fluorescent probe based on papain@AuNCs-Cu2+ complex has been constructed for sensitive, selective and "turn-on" detection of D-penicillamine (D-pen). The sensing probe has exhibited a remarkable fluorescence enhancement in the presence of D-pen, which can be measured ranging from 30.0 mu M to 2.0 mM (r(2) = 0.991) with the detection limit of 5.0 mu M. Furthermore, the developed assay has been utilized to explore the metabolic process of D-pen in rats after intraperitoneal injection. As far as we concerned, the present study has reported the first analytical method for sensing serum D-pen in real time by using the fluorescent papain@AuNCs-Cu2+ complex. Our strategy paves a way for digging up the biological and clinical application of the fluorescent AuNCs. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：133 / 139

页数：7

共 31 条

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