The cytochrome b6f complex at the crossroad of photosynthetic electron transport pathways

Regulation of photosynthetic electron transport at the level of the cytochrome b(6)f complex provides efficient performance of the chloroplast electron transport chain (ETC). In this review, after brief overview of the structural organization of the chloroplast ETC, the consideration of the problem of electron transport control is focused on the plastoquinone (PQ) turnover and its interaction with the b(6)f complex. The data available show that the rates of plastoquinol (PQH(2)) formation in PSII and its diffusion to the b(6)f complex do not limit the overall rate of electron transfer between photosystem II (PSII) and photosystem I (PSI). Analysis of experimental and theoretical data demonstrates that the rate-limiting step in the intersystem chain of electron transport is determined by PQH(2) oxidation at the Q(o)-site of the b(6)f complex, which is accompanied by the proton release into the thylakoid lumen. The acidification of the lumen causes deceleration of PQH(2) oxidation, thus impeding the intersystem electron transport. Two other mechanisms of regulation of the intersystem electron transport have been considered: (i) "state transitions" associated with the light-induced redistribution of solar energy between PSI and PSII, and (ii) redistribution of electron fluxes between alternative pathways (noncyclic electron transport and cyclic electron flow around PSI). (C) 2013 Elsevier Masson SAS. All rights reserved.