IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease

被引：5

作者：

Resende, Renata Goncalves ^{[1
]}

Correia-Silva, Jeane de Fatima ^{[2
]}

Silva, Tarcilia Aparecida ^{[3
]}

Salomao, Ulisses Eliezer ^{[4
]}

Marques-Silva, Luciano ^{[5
]}

Marciano Vieira, Erica Leandro ^{[6
]}

Dutra, Walderez Ornelas ^{[7
]}

Gomez, Ricardo Santiago ^{[3
]}

机构：

[1] Hosp Municipal Odilon Behrens, Serv Estomatol, BR-31110430 Belo Horizonte, MG, Brazil

[2] Ctr Univ Newton Paiva, Fac Odontol, BR-31230010 Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Dept Clin Patol & Cirurgia Odontol, Fac Odontol, BR-31270901 Belo Horizonte, MG, Brazil

[4] Univ Fed Minas Gerais, Dept Odontol Restauradora, Fac Odontol, BR-31270901 Belo Horizonte, MG, Brazil

[5] Ciodonto Univ FACSETE, BR-35700170 Sete Lagoas, MG, Brazil

[6] Univ Fed Minas Gerais, Fac Med, Lab Interdisciplinar Pesquisa Med, BR-3013010 Belo Horizonte, MG, Brazil

[7] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Morfol, BR-31270901 Belo Horizonte, MG, Brazil

来源：

MEDIATORS OF INFLAMMATION | 2014年 / 2014卷

关键词：

T-CELLS; INTERLEUKIN-17F GENES; TH17; CELLS; POLYMORPHISMS; DISTINCT; DIFFERENTIATION; ASSOCIATION; CYTOKINE; LINEAGE;

D O I：

10.1155/2014/571231

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Although interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet. The objective of this study was to investigate the association of IL17A and IL17F genes polymorphisms and IL-17A and IL-17F levels with cGVHD. IL-17A expression was also investigated in CD4(+) T cells of patients with systemic cGVHD. For Part I of the study, fifty-eight allo-HSCT recipients and donors were prospectively studied. Blood samples were obtained to determine IL17A and IL17F genes polymorphisms. Cytokines levels in blood and saliva were assessed by ELISA at days +35 and +100 after HSCT. In Part II, for the immunophenotypic evaluation, eight patients with systemic cGVHD were selected and the expression of IL-17A was evaluated. We found association between recipient AA genotype with systemic cGVHD. No association was observed between IL-17A levels and cGVHD. Lower IL-17A levels in the blood were associated with AA genotype. In flow cytometry analysis, decreased expression of IL-17A was observed in patients with cGVHD after stimulation. In conclusion, IL-17A may have an important role in the development of systemic cGVHD.

引用

页数：7

共 29 条

[1] Distinct roles for donor- and host-derived antigen-presenting cells and costimulatory molecules in murine chronic graft-versus-host disease: requirements depend on target organ [J].

Anderson, BE ;

McNiff, JM ;

Jain, D ;

Blazar, BR ;

Shlomchik, WD ;

Shlomchik, MJ .

BLOOD, 2005, 105 (05) :2227-2234

[2] The influence of polymorphisms of interleukin-17A and interleukin-17F genes on the susceptibility to ulcerative colitis [J].

Arisawa, Tomiyasu ;

Tahara, Tomomitsu ;

Shibata, Tomoyuki ;

Nagasaka, Mitsuo ;

Nakamura, Masakatsu ;

Kamiya, Yoshio ;

Fujita, Hiroshi ;

Nakamura, Masahiko ;

Yoshioka, Daisuke ;

Arima, Yuko ;

Okubo, Masaaki ;

Hirata, Ichiro ;

Nakano, Hiroshi .

JOURNAL OF CLINICAL IMMUNOLOGY, 2008, 28 (01) :44-49

[3] Advances in graft-versus-host disease biology and therapy [J].

Blazar, Bruce R. ;

Murphy, William J. ;

Abedi, Mehrdad .

NATURE REVIEWS IMMUNOLOGY, 2012, 12 (06) :443-458

[4] In vitro-differentiated TH17 cells mediate lethal acute graft-versus-host disease with severe cutaneous and pulmonary pathologic manifestations [J].

Carlson, Michael J. ;

West, Michelle L. ;

Coghill, James M. ;

Panoskaltsis-Mortari, Angela ;

Blazar, Bruce R. ;

Serody, Jonathan S. .

BLOOD, 2009, 113 (06) :1365-1374

[5] Interleukin 17 Is Not Required for Autoimmune-Mediated Pathologic Damage during Chronic Graft-versus-Host Disease [J].

Chen, Xiao ;

Das, Rupali ;

Komorowski, Richard ;

van Snick, Jacques ;

Uyttenhove, Catherine ;

Drobyski, William R. .

BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, 2010, 16 (01) :123-128

[6] Association between Polymorphisms in Interleukin-17A and-17F Genes and Chronic Periodontal Disease [J].

Correa, Joice Dias ;

Moreira Madeira, Mila Fernandes ;

Resende, Renata Goncalves ;

Correia-Silva, Jeane de Fatima ;

Gomez, Ricardo Santiago ;

de Souza, Danielle da Gloria ;

Teixeira, Mauro Martins ;

Queiroz-Junior, Celso Martins ;

da Silva, Tarcilia Aparecida .

MEDIATORS OF INFLAMMATION, 2012, 2012

[7]

Crawley E, 1999, ARTHRITIS RHEUM-US, V42, P1101, DOI 10.1002/1529-0131(199906)42:6<1101::AID-ANR6>3.0.CO

[8]

2-Y

[9] Interleukin-17-Producing T-Helper Cells as New Potential Player Mediating Graft-Versus-Host Disease in Patients Undergoing Allogeneic Stem-Cell Transplantation [J].

Dander, Erica ;

Balduzzi, Adriana ;

Zappa, Greta ;

Lucchini, Giovanna ;

Perseghin, Paolo ;

Andre, Valentina ;

Todisco, Elisabetta ;

Rahal, Daoud ;

Migliavacca, Maddalena ;

Longoni, Daniela ;

Solinas, Graziella ;

Villa, Antonello ;

Berti, Emilio ;

Della Mina, Pamela ;

Parma, Matteo ;

Allavena, Paola ;

Biagi, Ettore ;

Rovelli, Attilio ;

Biondi, Andrea ;

D'Amico, Giovanna .

TRANSPLANTATION, 2009, 88 (11) :1261-1272

[10] A single nucleotide polymorphism of IL-17 gene in the recipient is associated with acute GVHD after HLA-matched unrelated BMT [J].

Espinoza, J. L. ;

Takami, A. ;

Onizuka, M. ;

Kawase, T. ;

Sao, H. ;

Akiyama, H. ;

Miyamura, K. ;

Okamoto, S. ;

Inoue, M. ;

Ohtake, S. ;

Fukuda, T. ;

Morishima, Y. ;

Kodera, Y. ;

Nakao, S. .

BONE MARROW TRANSPLANTATION, 2011, 46 (11) :1455-1463

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