Measurement and meaning of cellular thiol:disufhide redox status

被引:55
作者
Comini, Marcelo A. [1 ]
机构
[1] Inst Pasteur Montevideo, Lab Redox Biol Trypanosomes, Mataojo 2020, Montevideo 11400, Uruguay
关键词
Glutaredoxin; glutathione; redox biosensor; redox signaling; thioredoxin; DISULFIDE BOND FORMATION; DIFFERENCE GEL-ELECTROPHORESIS; PROTEIN S-THIOLATION; FLUORESCENT-PROBE; OXIDATIVE STRESS; THIOREDOXIN REDUCTASE; ENDOPLASMIC-RETICULUM; CHEMILUMINESCENCE DETERMINATION; PHOSPHORESCENT CHEMODOSIMETER; MITOCHONDRIAL PROTEINS;
D O I
10.3109/10715762.2015.1110241
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The functional group of cysteine is a thiol group (SH) that, due to its chemical reactivity, is able to undergo a wide array of modifications each with the potential to confer a different property or function to the molecule harboring this residue. Most of these modifications involve the reversible oxidation of the thiol to sulfenic acid (SOH), and disulfide, including intra- and intermolecular disulfides between polypeptides and glutathione (glutathionylation). The reversibility of these oxidations allows thiol groups to serve as versatile chemical and structural transducing elements in several low molecular mass metabolites and proteins. A plethora of cellular functions such as DNA and protein synthesis, protein secretion, cytoskeleton architecture, differentiation, apoptosis, and anti-oxidant defense, are recognized to be modulated, at certain stage, by thiol-disulfide exchange mechanisms of redox active thiol groups. All organisms are equipped with enzymatic systems composed by NADPH-dependent reductases, redoxins, and peroxidases that provide kinetic control of global thiol-redox homeostasis as well as target selectivity. These redox systems are distributed in different subcellular compartments and are not in equilibrium with each other. In consequence, measuring cellular thiol-disulfide status represents a challenge for studies aimed to obtain dynamic and spatio-temporal resolution. This review provides a summary of the methods and tools available to quantify the thiol redox status of cells.
引用
收藏
页码:246 / 271
页数:26
相关论文
共 169 条
[1]   Redesign of Genetically Encoded Biosensors for Monitoring Mitochondrial Redox Status in a Broad Range of Model Eukaryotes [J].
Albrecht, Simone C. ;
Sobotta, Mirko C. ;
Bausewein, Daniela ;
Aller, Isabel ;
Hell, Ruediger ;
Dick, Tobias P. ;
Meyer, Andreas J. .
JOURNAL OF BIOMOLECULAR SCREENING, 2014, 19 (03) :379-386
[2]   In Vivo Mapping of Hydrogen Peroxide and Oxidized Glutathione Reveals Chemical and Regional Specificity of Redox Homeostasis [J].
Albrecht, Simone C. ;
Barata, Ana Gomes ;
Grosshans, Joerg ;
Teleman, Aurelio A. ;
Dick, Tobias P. .
CELL METABOLISM, 2011, 14 (06) :819-829
[3]   Development of roGFP2-derived redox probes for measurement of the glutathione redox potential in the cytosol of severely glutathione-deficient rml1 seedlings [J].
Aller, Isabel ;
Rouhier, Nicolas ;
Meyer, Andreas J. .
FRONTIERS IN PLANT SCIENCE, 2013, 4
[4]  
Antelmann H., 2015, Molecular Medical Microbiology, VSecond, P249, DOI [DOI 10.1016/B978-0-12-397169-2.00013-5, 10.1016/B978-0-12-397169-2.00013-5]
[5]   Thiol-Based Redox Switches and Gene Regulation [J].
Antelmann, Haike ;
Helmann, John D. .
ANTIOXIDANTS & REDOX SIGNALING, 2011, 14 (06) :1049-1063
[6]   An Atlas of the Thioredoxin Fold Class Reveals the Complexity of Function-Enabling Adaptations [J].
Atkinson, Holly J. ;
Babbitt, Patricia C. .
PLOS COMPUTATIONAL BIOLOGY, 2009, 5 (10)
[7]   Exploring real-time in vivo redox biology of developing and aging Caenorhabditis elegans [J].
Back, Patricia ;
De Vos, Winnok H. ;
Depuydt, Geert G. ;
Matthijssens, Filip ;
Vanfleteren, Jacques R. ;
Braeckman, Bart P. .
FREE RADICAL BIOLOGY AND MEDICINE, 2012, 52 (05) :850-859
[8]   Mass spectrometry in studies of protein thiol chemistry and signaling: Opportunities and caveats [J].
Baez, Nelmi O. Devarie ;
Reisz, Julie A. ;
Furdui, Cristina M. .
FREE RADICAL BIOLOGY AND MEDICINE, 2015, 80 :191-211
[9]   Redox outside the Box: Linking Extracellular Redox Remodeling with Intracellular Redox Metabolism [J].
Banerjee, Ruma .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (07) :4397-4402
[10]   Transient receptor potential melastatin 4 inhibition prevents lipopolysaccharide-induced endothelial cell death [J].
Becerra, Alvaro ;
Echeverria, Cesar ;
Varela, Diego ;
Sarmiento, Daniela ;
Armisen, Ricardo ;
Nunez-Villena, Felipe ;
Montecinos, Mario ;
Simon, Felipe .
CARDIOVASCULAR RESEARCH, 2011, 91 (04) :677-684