Rapid tests for the detection of the Mycobacterium abscessus subsp bolletii strain responsible for an epidemic of surgical-site infections in Brazil

被引：5

作者：

Matsumoto, Cristianne Kayoko ^{[1
]}

Chimara, Erica ^{[2
]}

Ramos, Jesus Pais ^{[3
]}

Dias Campos, Carlos Eduardo ^{[3
]}

de Souza Caldas, Paulo Cesar ^{[3
]}

Batista Lima, Karla Valeria ^{[4
]}

Lopes, Maria Luiza ^{[4
]}

Duarte, Rafael Silva ^{[5
]}

Leao, Sylvia Cardoso ^{[1
]}

机构：

[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, Sao Paulo, Brazil

[2] Adolfo Lutz Inst, Nucleo TB & Micobacterioses, Sao Paulo, Brazil

[3] Fiocruz MS, Ctr Referencia Prof Helio Fraga, Escola Nacl Saude Publ, Rio De Janeiro, RJ, Brazil

[4] Inst Evandro Chagas, Belem, PA, Brazil

[5] Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, Rio De Janeiro, RJ, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2012年 / 107卷 / 08期

基金：

巴西圣保罗研究基金会;

关键词：

Mycobacterium abscessus; epidemic; rapid test; rpoB; epidemiological surveillance; RIBOSOMAL-RNA; IDENTIFICATION; GENE; ALGORITHM; CHELONAE; HSP65; PCR;

D O I：

10.1590/S0074-02762012000800002

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A single strain of Mycobacterium abscessus subsp. bolletii, characterised by a particular rpoB sequevar and two highly related pulsed field gel electrophoresis patterns has been responsible for a nationwide outbreak of surgical infections in Brazil since 2004. In this study, we developed molecular tests based on polymerase chain reaction restriction-enzyme analysis (PRA) and sequencing for the rapid identification of this strain. Sequences of 15 DNA regions conserved in mycobacteria were retrieved from GenBank or sequenced and analysed in silico. Single nucleotide polymorphisms specific to the epidemic strain and located in enzyme recognition sites were detected in rpoB, the 3' region of the 16S rDNA and gyrB. The three tests that were developed, i.e., PRA-rpoB, PRA-16S and gyrB sequence analysis, showed 100%, 100% and 92.31% sensitivity and 93.06%, 90.28% and 100% specificity, respectively, for the discrimination of the surgical strain from other M. abscessus subsp. bolletii isolates, including 116 isolates from 95 patients, one environmental isolate and two type strains. The results of the three tests were stable, as shown by results obtained for different isolates from the same patient. In conclusion, due to the clinical and epidemiological importance of this strain, these tests could be implemented in reference laboratories for the rapid preliminary diagnosis and epidemiological surveillance of this epidemic strain.

引用

页码：969 / +

页数：11

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