Cytokine expression in gingival and intestinal tissues of patients with periodontitis and inflammatory bowel disease: An exploratory study

被引：32

作者：

Bittencourt Menegat, Juliana Santos ^{[1
]}

Lira-Junior, Ronaldo ^{[1
]}

Siqueira, Mariana Alves ^{[1
,2
]}

Brito, Fernanda ^{[1
]}

Carvalho, Ana Teresa ^{[3
]}

Fischer, Ricardo Guimaraes ^{[1
]}

Figueredo, Carlos Marcelo ^{[1
]}

机构：

[1] Univ Estado Rio De Janeiro, Fac Odontol, Dept Periodontol, Blvd 28 Setembro 157,2o Andar, BR-20551030 Rio De Janeiro, RJ, Brazil

[2] Univ Veiga de Almeida, Fac Odontol, Dept Periodontol, Rio De Janeiro, Brazil

[3] Univ Estado Rio De Janeiro, Fac Med, Dept Internal Med, Blvd 28 Setembro 157,2o Andar, BR-20551030 Rio De Janeiro, RJ, Brazil

来源：

ARCHIVES OF ORAL BIOLOGY | 2016年 / 66卷

关键词：

Periodontitis; Gingiva; Inflammatory bowel disease; Cytokines; ULCERATIVE-COLITIS; CROHNS-DISEASE; POTENTIAL ROLE; CELLS; RESPONSES; IL-33; IL-17; TH17; INDEX; TH2;

D O I：

10.1016/j.archoralbio.2016.02.018

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective: To evaluate the expression of the cytokines IFN-gamma, IL-1 beta, IL-4, IL-6, IL-10, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IL-17A, IL-17F, sCD40L, and TNF-alpha. in gingival tissue and intestinal mucosa of patients having both periodontitis and inflammatory bowel disease (IBD) and assess how they cluster in both tissues. Methods: This cross-sectional study selected 28 patients with periodontitis (18 with Crohn's disease and 10 with ulcerative colitis) from the IBD gastroenterology outpatient clinic at the Pedro Ernesto University Hospital. Patients were assessed using questionnaire, medical chart check and periodontal examination. Gingival and intestinal biopsies were collected and homogenized using a cell disruptor. Cytokines expression was evaluated through multiplex technology. Cluster analysis was performed based on cytokine's correlation strength and presented in dendrograms. Results: Crohn's disease and ulcerative colitis patients exhibited no significant difference between them in cytokine levels (p > 0.05), so they were analysed together. Significantly higher levels of IL-17A, IL-17F, IL-22, IL-25, IL-33, IL-10, and INF-gamma were found in gingival tissues in comparison with intestinal mucosa (p < 0.05). In gingival tissue, cytokines formed the following clusters: IL-25/IL-10/IL-33 (r=0.775), IL-22/IL-23/IL-6 (r = 0.681) and IL-6/IL-25/IL-33/IL-10 (r = 0.660). In intestinal mucosa, the following clusters were formed: IL-6/IL-21/IL-10 (r = 0.880), IL-17A/IL-6/IL-21/IL-10 (r = 0.826), IL-17F/IL-33/IL-25 (r=0.813) and IL-23/IL-2/IL-17A/IL-6/IL-21/IL-10 (r = 0.785). Conclusion: Expression of IL-17A, IL-17F, IL-22, IL-25, IL-33, IL-10, and INF-gamma was significantly increased in gingival tissue in comparison with intestinal mucosa of patients with periodontitis and IBD. The cytokine clustering pattern was different in gingival and intestinal tissues. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：141 / 146

页数：6

共 34 条

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