A novel phospholipase A2 from the venom glands of Bungarus candidus:: cloning and sequence-comparison

被引：8
作者：
Tsai, IH [1 ]
Hsu, HY
Wang, YM
机构：
[1] Acad Sinica, Inst Biol Chem, Taipei, Taiwan
[2] Natl Taiwan Univ, Coll Sci, Taipei 106, Taiwan
来源：
TOXICON | 2002年 / 40卷 / 09期
关键词：
Malayan krait (Bungarus candidus); snake venom; cDNA cloning; phospholipase A(2); phylogenetic analysis;
D O I：
10.1016/S0041-0101(02)00150-2
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The presence of phospholipase A(2) (PLA(2)) in the venom of Malayan krait (Bungarus candidus) and its structure were studied. The PLA(2) cDNAs from the venom gland of B. candidus (Indonesia origin) were amplified by the polymerase chain reactions (PCR) and cloned. The primers used were based on the cDNA sequences of several homologous B. multicinctus venom PLA(2)s. In addition to the A-chains of beta-bungarotoxins, a novel B. candidus PLA(2) was cloned and its full amino acid sequence deduced. Having totally 125 amino acid residues, the PLA(2) contains a pancreatic loop and is 61% identical to the acidic PLA(2) of king cobra venom. However, the enzyme was not detected from the venom sample. Its structural relationships to other elapid venom PLA(2)s were analyzed with a phylogenetic tree and discussed. (C) 2002 Elsevier Science Ltd. All rights reserved.
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收藏
页码：1363 / 1367
页数：5
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