Optimized ultrasound-assisted extraction of phenolic compounds from Thymus comosus Heuff. ex Griseb. et Schenk (wild thyme) and their bioactive potential

被引：37

作者：

Babota, Mihai ^{[1
]}

Frumuzachi, Oleg ^{[1
]}

Gavan, Alexandru ^{[2
]}

Iacovita, Cristian ^{[3
]}

Pinela, Jose ^{[4
]}

Barros, Lillian ^{[4
]}

Ferreira, Isabel C. F. R. ^{[4
]}

Zhang, Leilei ^{[5
]}

Lucini, Luigi ^{[5
]}

Rocchetti, Gabriele ^{[6
]}

Tanase, Corneliu ^{[7
]}

Crisan, Gianina ^{[1
]}

Mocan, Andrei ^{[1
,8
]}

机构：

[1] Iuliu Hatieganu Univ Med & Pharm, Dept Pharmaceut Bot, Gheorghe Marinescu St 23, Cluj Napoca 400337, Romania

[2] Iuliu Hatieganu Univ Med & Pharm, Dept Med Devices, 4 Louis Pasteur, Cluj Napoca 400349, Romania

[3] Iuliu Hatieganu Univ Med & Pharm, Dept Pharmaceut Phys Biophys, Louis Pasteur St 6, Cluj Napoca 400349, Romania

[4] Inst Politecn Braganca, Ctr Invest Montanha CIMO, Campus Santa Apolonia, P-5300253 Braganca, Portugal

[5] Univ Cattolica Sacro Cuore, Dept Sustainable Food Proc, Via Emilia Parmense 84, I-29122 Piacenza, Italy

[6] Univ Cattolica Sacro Cuore, Dept Anim Sci Food & Nutr, Via Emilia Parmense 84, I-29122 Piacenza, Italy

[7] George Emil Palade Univ Med Pharm Sci & Technol T, Dept Pharmaceut Bot, 38 Gheorghe Marinescu St, Targu Mures 540139, Romania

[8] Univ Agr Sci & Vet Med, Inst Adv Hort Res Transylvania, Lab Chromatog, Cluj Napoca 400372, Romania

来源：

ULTRASONICS SONOCHEMISTRY | 2022年 / 84卷

关键词：

Thymus comosus Heuff; ex Griseb; et Schenk; Ultrasound-assisted extraction; Optimisation; Design of Experiments; Phenolic compounds; ANTIOXIDANT CAPACITY; ESSENTIAL OIL; PROFILES; FOOD;

D O I：

10.1016/j.ultsonch.2022.105954

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

An optimized ultrasound-assisted extractive method was developed to obtain a polyphenol-enriched extract from the aerial parts of Thymus comosus Heuff. ex Griseb. et Schenk. Optimization process was conducted based on Design of Experiment (DoE) principles, determining the influence of three independent variables (time, ultrasound amplitude, ethanol concentration) on the total phenolic content of the extract (dependent variable). Additionally, the phenolic composition of the extract was characterized through UHPLC-HRMS, revealing beside the most abundant flavonoid-type compounds the presence of salvianolic acids C, D and L in high amounts. Phytochemical profile of the extract was correlated with its antioxidant activity (tested through five complementary assays) and enzyme-inhibitory potential, showing important antiglucosidase and anticholinesterase effects. Overall, it was concluded that the developed method is suitable for obtaining a good recovery of both phenolic and non-phenolic compounds from Thymus comosus aerial parts, and their presence in the optimized extract is responsible for its pharmacological potential.

引用

页数：10

共 59 条

[1] Edible packaging coating of encapsulated thyme essential oil in liposomal chitosan emulsions to improve the shelf life of Karish cheese [J].

Al-Moghazy, Marwa ;

El-sayed, Hoda S. ;

Salama, Heba H. ;

Nada, Ahmed A. .

FOOD BIOSCIENCE, 2021, 43

[2] Extraction, optimisation and characterisation of phenolics from Thymus vulgaris L.: phenolic content and profiles in relation to antioxidant, antidiabetic and antihypertensive properties [J].

Alu'datt, Muhammad H. ;

Rababah, Taha ;

Johargy, Ayman ;

Gammoh, Sana ;

Ereifej, Khalil ;

Alhamad, Mohammad N. ;

Brewer, Mary Susan ;

Saati, Abdullah A. ;

Kubow, Stan ;

Rawshdeh, Mervat .

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2016, 51 (03) :720-730

[3] Green Extraction Methods for Polyphenols from Plant Matrices and Their Byproducts: A Review [J].

Ameer, Kashif ;

Shahbaz, Hafiz Muhammad ;

Kwon, Joong-Ho .

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2017, 16 (02) :295-315

[4] Extraction of Aloesin from Aloe vera Rind Using Alternative Green Solvents: Process Optimization and Biological Activity Assessment [J].

Anibarro-Ortega, Mikel ;

Pinela, Jose ;

Ciric, Ana ;

Lopes, Elsa ;

Molina, Adriana K. ;

Calhelha, Ricardo C. ;

Sokovic, Marina ;

Ferreira, Olga ;

Ferreira, Isabel C. F. R. ;

Barros, Lillian .

BIOLOGY-BASEL, 2021, 10 (10)

[5]

[Anonymous], 2017, PHEUR, V9, P1638

[6]

[Anonymous], 2015, MODDE 110

[7] Statistically Designed Extraction of Herbs Using Ultrasound Waves: A Review [J].

Arya, Ashwani ;

Chahal, Rubal ;

Nanda, Arun ;

Kaushik, Deepak ;

Bin-Jumah, May ;

Rahman, Md. Habibur ;

Abdel-Daim, Mohamed M. ;

Mittal, Vineet .

CURRENT PHARMACEUTICAL DESIGN, 2021, 27 (34) :3638-3655

[8] Chemical Composition, Diuretic, and Antityrosinase Activity of Traditionally Used Romanian Cerasorum stipites [J].

Babota, Mihai ;

Vostinaru, Oliviu ;

Paltinean, Ramona ;

Mihali, Cosmin ;

Dias, Maria Ines ;

Barros, Lillian ;

Ferreira, Isabel C. F. R. ;

Mocan, Andrei ;

Crisan, Ovidiu ;

Nicula, Cristina ;

Crisan, Gianina .

FRONTIERS IN PHARMACOLOGY, 2021, 12

[9] Phytochemical Analysis, Antioxidant and Antimicrobial Activities of Helichrysum arenarium (L.) Moench. and Antennaria dioica (L.) Gaertn. Flowers [J].

Babota, Mihai ;

Mocan, Andrei ;

Vlase, Laurian ;

Crisan, Ovidiu ;

Ielciu, Irina ;

Gheldiu, Ana-Maria ;

Vodnar, Dan Cristian ;

Crisan, Gianina ;

Paltinean, Ramona .

MOLECULES, 2018, 23 (02)

[10] Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts [J].

Boutaoui, Nassima ;

Zaiter, Lahcene ;

Benayache, Fadila ;

Benayache, Samir ;

Carradori, Simone ;

Cesa, Stefania ;

Giusti, Anna Maria ;

Campestre, Cristina ;

Menghini, Luigi ;

Innosa, Denise ;

Locatelli, Marcello .

MOLECULES, 2018, 23 (02)

← 1 2 3 4 5 6 →