The effect of strontium modified rough titanium surface on biologic response of MC3T3-E1 cells

被引:11
作者
Jiang, Huanhuan [1 ]
Zhou, Wenjuan [2 ]
Wang, Binchen [2 ]
Tang, Liqin [1 ]
机构
[1] Wuxi Stomatol Hosp, Dept Dent Implantol, 6 Jiankang Rd, Wuxi, Jiangsu, Peoples R China
[2] Yantai Stomatol Hosp, Dept Dent Implantol, 142 North Great St, Zhifu Dist, Yantai, Peoples R China
关键词
Strontium; Titanium; Surface modification; Magnetron sputtering; Osteoblast; HYDROXYAPATITE COATINGS; OSTEOBLAST RESPONSE; OXIDE LAYER; BONE; OSSEOINTEGRATION; DEPOSITION; RANELATE; IMPLANTS; ACTIVATION; RELEASE;
D O I
10.4012/dmj.2019-188
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
It has been shown that strontium (Sr) promotes bone formation, reduces bone resorption. In the study, magnetron sputtering method was used for preparing Sr covered on sandblasted and acid-etched (SLA) titanium surface. Surface morphology was examined by scanning electron microscopy (SEM), chemical composition of surface was investigated by X-ray energy dispersive spectrometry (EDS). MC3T3-Elcells were cultured on Sr-SLA and SLA disks. Cell morphology was studied through confocal laser scanning microscope and SEM. Cell proliferation were measured by MTT assay. Degrees of mineralization and alkaline phosphatase (ALP) activity were calculated and compared. EDS and SEM results indicated that Sr ions were successfully loaded in Sr-SLA surface, whereas, Sr-SLA and SLA surfaces demonstrated similar typical isotropic irregular indentations. The MC3T3-E1 cells developed on Sr-SLA surface showed improved morphology, better proliferation as well as greater differentiation.These findings suggest that the modification with Sr incorporated in moderately rough surface has a favorable biocompatibility.
引用
收藏
页码:808 / 814
页数:7
相关论文
共 50 条
[21]   The response of osteoblastic MC3T3-E1 cells to micro- and nano-textured, hydrophilic and bioactive titanium surfaces [J].
Lumetti, S. ;
Manfredi, E. ;
Ferraris, S. ;
Spriano, S. ;
Passeri, G. ;
Ghiacci, G. ;
Macaluso, G. ;
Galli, C. .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2016, 27 (04) :1-9
[22]   Differential Response of MC3T3-E1 and Human Mesenchymal Stem Cells to Inositol Hexakisphosphate [J].
del Mar Arriero, Maria ;
Ramis, Joana M. ;
Perello, Joan ;
Monjo, Marta .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2012, 30 (04) :974-986
[23]   Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1) [J].
Pramono, Samdharu ;
Pugdee, Kamotparn ;
Suwanprateep, Jintamai ;
Koontongkaew, Sittichai .
JOURNAL OF DENTAL SCIENCES, 2016, 11 (04) :427-436
[24]   Enhanced initial proliferation and differentiation of MC3T3-E1 cells on HF/HNO3 solution treated nanostructural titanium surface [J].
He, Fuming ;
Zhang, Feng ;
Yang, Guoli ;
Wang, Xiaoxiang ;
Zhao, Shifang .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 2010, 110 (04) :E13-E22
[25]   Effect of the micro/nanostructured topography of polyetheretherketone on the behavior of MC3T3-E1 preosteoblasts [J].
Zhao, Yuqing ;
Wang, Jing ;
Wu, Fan ;
Liu, Lipeng ;
Wu, Gaoyi ;
Zhou, Libo .
JOURNAL OF APPLIED POLYMER SCIENCE, 2024, 141 (15)
[26]   Ultrasonic irradiation assisted surface modification of titanium plates to improve MC3T3-E1 cell proliferation [J].
Tan, Wanye ;
Zhao, Hongshi ;
Ren, Na ;
Li, Jianhua ;
Li, Guohong ;
Wang, Guancong ;
Wei, Fengcai ;
Boughton, Robert I. ;
Liu, Hong .
ULTRASONICS SONOCHEMISTRY, 2013, 20 (01) :216-221
[27]   The Effect of Selenium Nanoparticles on the Osteogenic Differentiation of MC3T3-E1 Cells [J].
Lee, Sang-Cheol ;
Lee, Na-Hyun ;
Patel, Kapil D. ;
Jang, Tae-Su ;
Knowles, Jonathan Campbell ;
Kim, Hae-Won ;
Lee, Hae-Hyoung ;
Lee, Jung-Hwan .
NANOMATERIALS, 2021, 11 (02) :1-15
[28]   Effect of Azithromycin on Mineralized Nodule Formation in MC3T3-E1 Cells [J].
Kato, Kengo ;
Ozaki, Manami ;
Nakai, Kumiko ;
Nagasaki, Maki ;
Nakajima, Junya ;
Koshi, Ryosuke ;
Tanaka, Hideki ;
Kawato, Takayuki ;
Tonogi, Morio .
CURRENT ISSUES IN MOLECULAR BIOLOGY, 2021, 43 (03) :1451-1459
[29]   A chimeric peptide that binds to titanium and mediates MC3T3-E1 cell adhesion [J].
Wang, Dan ;
Mao, Jing ;
Zhou, Bin ;
Liao, Xiao-Fu ;
Gong, Shi-Qiang ;
Liu, Yan ;
Zhang, Jing-Tao .
BIOTECHNOLOGY LETTERS, 2011, 33 (01) :191-197
[30]   Effect of acetaminophen on osteoblastic differentiation and migration of MC3T3-E1 cells [J].
Yoshihiro Nakatsu ;
Fumio Nakagawa ;
Sen Higashi ;
Tomoko Ohsumi ;
Shunji Shiiba ;
Seiji Watanabe ;
Hiroshi Takeuchi .
Pharmacological Reports, 2018, 70 :29-36