Prodigiosin-Conjugated Aptamer for Attachment to the Surface of Brain Cancer Cells Mediated by Glutamate Receptor

被引：7

作者：

Ayatollahi, Mohammadagha ^{[1
,2
,3
]}

Ayatollahi, Gelayol ^{[4
]}

Rashidi, Mohsen ^{[5
]}

Hekmatimoghaddam, Seyedhossein ^{[6
]}

Mosshafi, Mohammadhassan ^{[1
]}

Jebali, Ali ^{[6
]}

Iman, Maryam ^{[7
]}

Sardo, Hossein Shahdadi ^{[8
]}

机构：

[1] Kerman Univ Med Sci, Pharmaceut Res Ctr, Inst Neuropharmacol, Kerman, Iran

[2] Yazd Univ Med Sci, Sch Pharm, Dept Pharmacol & Toxicol, Yazd, Iran

[3] Yazd Univ Med Sci, Sch Med, Dept Pharmacol & Toxicol, Yazd, Iran

[4] Shahid Sadoughi Univ Med Sci, Int Campus, Yazd, Iran

[5] Mazandaran Univ Med Sci, Fac Med, Dept Physiol & Pharmacol, Sari, Iran

[6] Shahid Sadoughi Univ Med Sci, Sch Paramed, Dept Lab Sci, Yazd, Iran

[7] Baqiyatallah Univ Med Sci, Chem Injuries Res Ctr, Syst Biol & Poisonings Inst, Tehran, Iran

[8] Mashhad Univ Med Sci, Targeted Drug Delivery Res Ctr, Sch Pharm, Mashhad, Iran

来源：

COLLOID AND INTERFACE SCIENCE COMMUNICATIONS | 2018年 / 24卷

关键词：

Prodigiosin; Aptamer; Brain cancer cells; Glutamate receptor; DRUG-DELIVERY; GOLD NANOPARTICLES; THERAPEUTICS; LIPOSOMES;

D O I：

10.1016/j.colcom.2018.03.006

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The aim of this study was to introduce a prodigiosin-conjugated aptamer to attach to surface of brain cancer cells. Crystal structure of glutamate receptor and 106 nucleotide aptamers were separately interacted in NVT ensemble for duration of 50,000 ps at 310 K by Ascalaph designer software. Finally, delta intermolecular energy (Delta INME) was separately obtained for each aptamer. For confirmation of simulation data, both brain cancer cells and normal brain cells were cultured, and separately incubated with the best selected aptamers. Finally, the adsorption percent of each aptamer was measured for both kinds of cells. Prodigiosin-conjugated aptamer 8, 10, 11, 23, and 69 could attach to all epitopes with high affinity and low Delta INME. In vitro test showed that all aptamers could bind to brain cancer cells more than normal cells. Moreover, aptamer 10 had the highest adsorption to brain cancer cells and the least adsorption to normal brain cells.

引用

页码：45 / 48

页数：4

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