The in vitro effects of sodium salicylate on von Willebrand factor and C-reactive protein production by endothelial cells

Objective The role of inflammatory and endothelial dysfunction markers in the atherogenic process has been well recognized. The data have made both C-reactive protein (CRP) and von Willebrand factor (vWF) promising targets for the cardiovascular disease research and drug development. Inhibition of CRP and vWF synthesis, therefore, might be a potential therapeutic strategy. Methods The effect of sodium salicylate on vWF production by human umbilical vein endothelial cells (HUVECs) using enzyme-linked immunosorbent Assays (ELISA) and real-time PCR was examined. In addition, small interfering RNA (siRNA) against NF-kappa B was used to investigate the existence of a role for this signaling pathway. Results Our findings demonstrated that sodium salicylate decreased vWF, but not CRP production at both mRNA and protein levels significantly and this might not occur via nuclear transcription factor (NF-kappa B) inhibition. Conclusion Our results indicated a further rationalization of the effects of sodium salicylate on atherothrombotic events by attenuation of vWF production.