Displacement Statistics of Unhindered Single Molecules Show noEnhanced Diffusion in Enzymatic Reactions

被引：16

作者：

Choi, Alexander A. ^{[1
]}

Park, Ha H. ^{[1
]}

Chen, Kun ^{[1
]}

Yan, Rui ^{[1
]}

Li, Wan ^{[1
]}

Xu, Ke ^{[1
,2
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Chan Zuckerberg Biohub, San Francisco, CA 94158 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2022年 / 144卷 / 11期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

ENHANCED DIFFUSION;

D O I：

10.1021/jacs.1c12328

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Recent studies have sparked debate over whethercatalytic reactions enhance the diffusion coefficientsDof enzymes.Through high statistics of the transient (600 mu s) displacements ofunhindered single molecules freely diffusing in common buffers, wehere quantifyDfor four enzymes under catalytic turnovers. Wethus formulate how similar to +/- 1% precisions may be achieved forD, andshow no changes in diffusivity for catalase, urease, aldolase, andalkaline phosphatase under the application of wide concentrationranges of substrates. Our single-molecule approach thus overcomespotential limitations and artifacts underscored by recent studies toshow no enhanced diffusion in enzymatic reactions

引用

页码：4839 / 4844

页数：6

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