Engineering the Stereochemistry of Cephalosporin for Specific Detection of Pathogenic Carbapenemase-Expressing Bacteria

被引：40

作者：

Shi, Haibin ^{[1
,2
]}

Cheng, Yunfeng ^{[1
,2
]}

Lee, Kyung Hyun ^{[1
,2
]}

Luo, Robert F. ^{[3
,4
]}

Banaei, Niaz ^{[3
,4
]}

Rao, Jianghong ^{[1
,2
]}

机构：

[1] Stanford Univ, Sch Med, Mol Imaging Program Stanford, Dept Radiol, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Mol Imaging Program Stanford, Dept Chem, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA

[4] Stanford Hosp & Clin, Clin Microbiol Lab, Palo Alto, CA 94304 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2014年 / 53卷 / 31期

关键词：

enzymes; fluorescence; fluorescent probes; metalloenzymes; stereochemistry; DESORPTION IONIZATION-TIME; BETA-LACTAMASE; PSEUDOMONAS-SPP; RAPID DETECTION; MULTIPLEX PCR; TUBERCULOSIS; IDENTIFICATION; INHIBITION; RESISTANCE;

D O I：

10.1002/anie.201402012

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Reported herein is the design of fluorogenic probes specific for carbapenem-resistant Enterobacteriaceae (CRE) and they were designed based on stereochemically modified cephalosporin having a 6,7-trans configuration. Through experiments using recombinant beta-lactamase enzymes and live bacterial species, these probes demonstrate the potential for use in the specific detection of carbapenemases, including metallo-beta-lactamases in active bacterial pathogens.

引用

页码：8113 / 8116

页数：4