Facile fabrication of an aptasensor for thrombin based on graphitic carbon nitride/TiO2 with high visible-light photoelectrochemical activity

被引:80
作者
Fan, Dawei [1 ]
Guo, Cuijuan [1 ]
Ma, Hongmin [1 ]
Zhao, Di [1 ]
Li, Yina [1 ]
Wu, Dan [1 ]
Wei, Qin [1 ]
机构
[1] Univ Jinan, Sch Chem & Chem Engn, Key Lab Chem Sensing & Anal Univ Shandong, Jinan 250022, Peoples R China
基金
中国国家自然科学基金;
关键词
Aptasensor; g-C3N4/TiO2; Thrombin; Visible-light; Photoelectrochemical activity; ENHANCED PHOTOCATALYTIC ACTIVITY; TITANIUM-DIOXIDE; DOPED TIO2; CELLS; GRAPHENE; G-C3N4; PHOTODEGRADATION; IRRADIATION; NANOSHEETS; HYBRID;
D O I
10.1016/j.bios.2015.08.029
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A novel aptasensor for thrombin with high visible-light activity was facilely fabricated based on graphitic carbon nitride/TiO2 (g-C3N4/TiO2) photoelectrochemical (PEC) composite. Crystallization of TiO2 nanoparticles (NPs) and their strong interaction with g-C3N4 sheet were confirmed by high-resolution transmission electron microscope (HR-TEM), both of which contributed to the high photocurrent intensity under visible-light irradiation. Carboxyl functionalized thrombin aptamers were first successfully bound to the g-C3N4/TiO2 modified electrode as proven by photoelectrochemical test and electrochemical impedance spectroscopy (EIS) analysis. Ascorbic acid was utilized as the electron donor for scavenging photo-generated holes and inhibiting light driven electron-hole pair recombination. The specific recognition between thrombin aptamer and thrombin led to the linear decrease of photocurrent with the increase of logarithm of thrombin concentration in the range of 5.0 x 10(-13) mol L-1 to 5.0 x 10(-9) mol L-1 with a detection limit of 1.2 x 10(-13) mol L-1. This proposed low-cost, convenient and sensitive aptasensor showed promising applications in biosensor and photoelectrochemical analysis. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:116 / 122
页数:7
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