EFFECT OF pH AND TEMPERATURE ON JUMBO SQUID PROTEINS

被引:10
作者
De la Fuente-Betancourt, G. [1 ]
Garcia-Carreno, F. [1 ]
Navarrete Del Toro, M. A. [1 ]
Cordova-Murueta, J. H. [1 ]
机构
[1] Ctr Invest Biol Noroeste CIBNOR, La Paz 23090, Bcs, Mexico
关键词
FUNCTIONAL-PROPERTIES; MUSCLE PROTEINS; EMULSIONS; GELATION; ALBUMIN; HEAT;
D O I
10.1111/j.1745-4514.2009.00216.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Evaluation of the effect of pH (2 to 13) and temperature (0 to 50C) on functional properties of jumbo squid proteins was performed, followed by a 2 x 3 factorial design for producing squid protein hydrolysates bearing useful functional properties. In particular, the effects of pH (8, 9 and 10) and temperature (30, 35, 40C) were evaluated. Alcalase and papain were tested on each treatment. The protein recovery, whippability and emulsifying capacity of the hydrolysates were evaluated. Almost 80% of the proteins were recovered in water-soluble form after hydrolysis with papain at pH 10 at the three temperatures. The highest values of whippability (245 +/- 17.7%), foam stability (100%), emulsion-forming capacity (27 +/- 0.97%) and stability (99.99 +/- 8.8%) occurred with papain-produced hydrolysates. When squid protein was treated at 50C and pH 8, the highest whippability value (390.0 +/- 0.1%) and foam stability (100%) were obtained when no enzyme was added.
引用
收藏
页码:260 / 272
页数:13
相关论文
共 22 条
[1]  
Adler-Nissen J, 1986, ENZYMIC HYDROLYSIS F
[2]  
[Anonymous], 1995, Official Method of Analysis, V16th
[3]  
DELTORO MAN, 2002, CURRENT PROTOCOLS S, V4, pCHB2
[4]  
GILDBERG A, 1993, PROCESS BIOCHEM, V28, P1, DOI 10.1016/0032-9592(94)80030-8
[5]   Functional and thermal gelation properties of squid mantle proteins affected by chilled and frozen storage [J].
Gómez-Guillén, MC ;
Martínez-Alvarez, O ;
Montero, P .
JOURNAL OF FOOD SCIENCE, 2003, 68 (06) :1962-1967
[6]   Dry-pasteurization of egg albumen powder in a fluidized bed. II. Effect on functional properties: gelation and foaming [J].
Hammershoj, M ;
Rasmussen, HC ;
Carstens, JH ;
Pedersen, H .
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2006, 41 (03) :263-274
[7]  
HASHIMOTO K, 1979, B JPN SOC SCI FISH, V45, P1435
[8]  
HILL SE, 1996, METHODS TESTING PROT
[9]   CHARACTERIZATION OF THE KINETICS OF BREAKDOWN OF PROTEIN STABILIZED OIL IN WATER EMULSIONS [J].
JACKMAN, RL ;
YADA, RY ;
PAULSON, AT .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1989, 37 (03) :600-604
[10]   Fish protein hydrolysates: Production, biochemical, and functional properties [J].
Kristinsson, HG ;
Rasco, BA .
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 2000, 40 (01) :43-81