Nanofibers are produces from organic and inorganic polymers via electrospnning technology. Both polymer solutions and polymer melts can be electrospun. Fiber diameters of 50 to 500 nanometers are typical. An industrial scale production method of nanofibres production has been developed. Small fibre diameters and great specific surface are the main specific properties of nanofiber assemblies, namely nanofiber layers. Number of specific end-uses of nanofibres have been developed such as filters, sound absorbing materials, wound dressings, scaffolds for tissue engineering etc. Machinery for nanofibres production is produced and offered in the Czech Republic. There is a great potential for utilization of nanofibres in civil engineering.