High-resolution phenotypic profiling of natural productsinduced effects on the single-cell level

被引：15
|
作者：
Kremb, Stephan [1 ]
Voolstra, Christian R. [1 ]
机构：
[1] King Abdullah Univ Sci & Technol, Red Sea Res Ctr, Div Biol & Environm Sci & Engn BESE, Thuwal 239556900, Saudi Arabia
来源：
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词：
DRUG DISCOVERY; TOPOISOMERASE-II; PRODUCT; ASSAY; CYTOTOXICITY; ANNOTATION; MECHANISM; LIBRARIES;
D O I：
10.1038/srep44472
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Natural products (NPs) are highly evolved molecules making them a valuable resource for new therapeutics. Here we demonstrate the usefulness of broad-spectrum phenotypic profiling of NPinduced perturbations on single cells with imaging-based High-Content Screening to inform on physiology, mechanisms-of-actions, and multi-level toxicity. Our technology platform aims at broad applicability using a comprehensive marker panel with standardized settings streamlined towards an easy implementation in laboratories dedicated to natural products research.
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页数：8
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