Expression, purification and preliminary X-ray crystallographic analysis of UDP-galactopyranose mutase from Deinococcus radiodurans

被引：4

作者：

Partha, Sarathy Karunan ^{[1
]}

Bonderoff, Sara A. ^{[1
]}

van Straaten, Karin E. ^{[1
]}

Sanders, David A. R. ^{[1
]}

机构：

[1] Univ Saskatchewan, Dept Chem, Saskatoon, SK S7N 5C9, Canada

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2009年 / 65卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

MOLECULAR REPLACEMENT; MYCOBACTERIA; TUBERCULOSIS; GROWTH;

D O I：

10.1107/S1744309109027754

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

UDP-galactopyranose mutase (UGM) catalyzes the interconversion of UDP-galactopyranose and UDP-galactofuranose. A UGM-substrate complex from Deinococccus radiodurans has been expressed, purified and crystallized. Crystals were obtained by the microbatch-under-oil method at room temperature. The crystals diffracted to 2.36 angstrom resolution at the Canadian Light Source The space group was found to be P2(1)2(1)2(1), with unit-cell parameters a = 134.0, b = 176.6, c = 221.6 angstrom. The initial structure solution was determined by molecular replacement using UGM from Mycobacterium tuberculosis (PDB code 1v0j) as a template model.

引用

页码：843 / 845

页数：3

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