Investigation of Natural Phosphatidylcholine Sources: Separation and Identification by Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry (LC-ESI-MS2) of Molecular Species

被引：92
作者：
Le Grandois, Julie [1 ]
Marchioni, Eric [1 ]
Zhao, Minjie [1 ]
Giuffrida, Francesca [2 ]
Ennahar, Said [1 ]
Bindler, Francoise [1 ]
机构：
[1] Univ Strasbourg, Lab Chim Analyt & Sci Aliment, IPHC, DSA,CNRS, F-67400 Illkirch Graffenstaden, France
[2] Nestec Ltd, Nestle Res Ctr, CH-1000 Lausanne, Switzerland
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2009年 / 57卷 / 14期
关键词：
Phosphatidylcholine; PUFA; supplementation; molecular species; lithium; LC-ESI-MS; POLYUNSATURATED FATTY-ACIDS; DOCOSAHEXAENOIC ACID; TERM INFANTS; ARACHIDONIC-ACID; PHOSPHOLIPIDS; BRAIN; HYDROXYLATION; FORMULA; HEALTHY; ACUITY;
D O I：
10.1021/jf900903e
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
This study is a contribution to the exploration of natural phospholipid (PL) sources rich in long-chain polyunsaturated fatty acids (LC-PUFAs) with nutritional interest. Phosphatidylcholines (PCs) were purified from total lipid extracts of different food matrices, and their molecular species were separated and identified by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS2). Fragmentation of lithiated adducts allowed for the identification of fatty acids linked to the glycerol backbone. Soy PC was particularly rich in species containing essential fatty acids, such as (18:2-18:2)PC (34.0%), (16:0-18:2)PC (20.8%), and (18:1-18:2)PC (16.3%). PC from animal sources (ox liver and egg yolk) contained major molecular species, such as (16:0-18:2) PC, (16:0-18:1)PC, (18:0-18:2)PC, or (18:0-18:1)PC. Finally, marine source (krill oil), which was particularly rich in (16:0-20:5)PC and (16:0-22:6)PC, appeared to be an interesting potential source for food supplementation with LC-PUFA-PLs, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).
引用
收藏
页码：6014 / 6020
页数：7
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