GhN/AINV13 positively regulates cotton stress tolerance by interacting with the 14-3-3 protein

被引：12

作者：

Chen, Baizhi ^{[1
]}

Wang, Xiaoyan ^{[2
]}

Lv, Jiaoyan ^{[1
]}

Ge, Mengjie ^{[3
]}

Qiao, Kaikai ^{[1
]}

Chen, Quanjia ^{[3
]}

Zhang, Kunpeng ^{[2
]}

Wang, Jingshun ^{[2
]}

Fan, Shuli ^{[1
]}

Ma, Qifeng ^{[1
]}

机构：

[1] Minist Agr, Key Lab Cotton Genet Improvement, Inst Cotton Res CAAS, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China

[2] Anyang Inst Technol, Coll Biol & Food Engn, Anyang 455000, Henan, Peoples R China

[3] Xinjiang Agr Univ, Xinjiang Res Base, State Key Lab Cotton Biol, Urumqi 830052, Xinjiang, Peoples R China

来源：

GENOMICS | 2021年 / 113卷 / 01期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

Neutral/alkaline invertases; Stress tolerance; Gene family; INVERTASE GENE FAMILY; VACUOLAR INVERTASE; ALKALINE/NEUTRAL-INVERTASE; NEUTRAL INVERTASE; CYTOSOLIC INVERTASE; SUGAR METABOLISM; FIBER INITIATION; SUCROSE; ROOT; EXPRESSION;

D O I：

10.1016/j.ygeno.2020.11.026

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Neutral/alkaline invertases (N/AINVs) are sucrose hydrolases with important roles in plants. In this study, 15, 15, 15, 29, and 30 N/AINVs were identified in the Gossypium species, G. raimondii, G. herbaceum, G. arboreum, G. hirsutum, and G. barbadense, respectively. Along with two previously discovered branches, alpha and beta, a new clade gamma was first discovered in our study. Investigation of gene collinearity showed that whole-genome duplication (WGD) and polyploidization were responsible for the expansion of the N/AINV gene family in allopolyploid Gossypium. Moreover, expression patterns revealed that GhN/AINV3/13/17/23/24/28 from the beta clade is highly expressed during the period of fiber initiation. The invertase activity of GhN/AINV13 and GhN/AINV23 were confirmed by restoring defects of invertase-deficient yeast mutant SEY2102. Treatments of abiotic stress showed that most GhN/AINVs were induced in response to polyethylene glycol (PEG) or salt stress. A virus-induced gene silencing (VIGS) experiment and yeast two-hybrid assay demonstrated that GhN/AINV13 may interact with their positive regulators Gh14-3-3 proteins and participate in the fiber initiation or stress tolerance of cotton. Our results provided fundamental information regarding N/AINVs and highlight their potential functions in cotton stress tolerance.

引用

页码：44 / 56

页数：13

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