Analysis of tamoxifen-DNA adducts in endometrial explants by MS and 32P-postlabeling

被引：15

作者：

Beland, FA ^{[1
]}

Churchwell, MI

Hewer, A

Phillips, DH

da Costa, GG

Marques, MM

机构：

[1] Natl Ctr Toxicol Res, Div Biochem Toxicol, Jefferson, AR 72079 USA

[2] Inst Canc Res, Sutton SM2 5NG, Surrey, England

[3] Univ Tecn Lisboa, Ctr Quim Estrutural, Inst Super Tecn, P-104900 Lisbon, Portugal

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 320卷 / 02期

关键词：

tamoxifen; P-32-postlabeling; HPLC; electrospray ionization tandem mass spectrometry; DNA adducts; endometrial cancer;

D O I：

10.1016/j.bbrc.2004.05.168

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nonsteroidal antiestrogen tamoxifen increases the risk of endometrial cancer; however, the mechanism for the induction of these tumors is not known. Recently, Sharma et a]. [Biochem. Biophys. Res. Commun. 307 (2003) 157], using high performance liquid chromatography (HPLC) with online postcolumn photochemical activation and fluorescence detection, reported the presence of (E)-alpha-(deoxyguanosin-N-2-yl)tamoxifen in DNA from human endometrial explants incubated with tamoxifen. Inasmuch as the methodology used by these investigators does not allow unambiguous characterization of tamoxifen-DNA adducts, we have used two additional techniques (HPLC coupled with electrospray ionization tandem mass spectrometry and P-32-postlabeling analyses) to assay for the presence of tamoxifen-DNA adducts in the human endometrial explant DNA. Tamoxifen-DNA adducts were not detected by either method. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：297 / 302

页数：6

共 50 条

[31] In vitro and in vivo P-32-postlabeling analysis of 4-vinyl-1-cyclohexene (butadiene dimer) diepoxide-DNA adducts
Randerath, K
Mabon, N
CANCER LETTERS, 1996, 101 (01) : 67 - 72
[32] P-32 POSTLABELING ANALYSIS OF DNA-ADDUCTS IN WHITE BLOOD-CELLS OF HUMANS EXPOSED TO RESIDENTIAL WOOD COMBUSTION PARTICULATE MATTER
HEUSSEN, GAH
BOUMAN, HGM
ROGGEBAND, R
BAAN, RA
ALINK, GM
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1994, 23 (02) : 121 - 127
[33] Analysis of 13 32P-DNA postlabeling studies on occupational cohorts exposed to air pollution
Peluso, M
Ceppi, M
Munnia, A
Puntoni, R
Parodi, S
AMERICAN JOURNAL OF EPIDEMIOLOGY, 2001, 153 (06) : 546 - 558
[34] IMMUNOAFFINITY CONCENTRATION OF HUMAN LUNG DNA-ADDUCTS USING AN ANTI-BENZO[A]PYRENE-DIOL-EPOXIDE-DNA ANTIBODY - ANALYSIS BY P-32 POSTLABELING OR ELISA
KING, MM
CUZICK, J
JENKINS, D
ROUTLEDGE, MN
GARNER, RC
MUTATION RESEARCH, 1993, 292 (02): : 113 - 122
[35] BULKY ADDUCTS DETECTED BY P-32 POSTLABELING IN DNA MODIFIED BY OXIDATIVE DAMAGE INVITRO - COMPARISON WITH RAT LUNG I-COMPOUNDS
RANDERATH, K
YANG, PF
DANNA, TF
REDDY, R
WATSON, WP
RANDERATH, E
MUTATION RESEARCH, 1991, 250 (1-2): : 135 - 144
[36] 32P-postlabelling analysis of 1,3-butadiene-induced DNA adducts in vivo and in vitro
Zhao, CY
Koskinen, M
Hemminki, K
BIOMARKERS, 2000, 5 (03) : 168 - 181
[37] P-32-postlabeling of 1,3-butadiene and 4-vinyl-1-cyclohexene metabolite DNA adducts: In vitro and in vivo applications
Mabon, N
Randerath, K
TOXICOLOGY, 1996, 113 (1-3) : 341 - 344
[38] Comparative P-32-postlabeling analysis of exogenous and endogenous DNA adducts in mouse skin exposed to a wood-preserving waste extract, a complex mixture of polycyclic and polychlorinated chemicals
Randerath, K
Zhou, GD
Randerath, E
Safe, SH
Donnelly, KC
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1997, 29 (04) : 372 - 378
[39] EXOGENOUS AND ENDOGENOUS DNA MODIFICATIONS AS MONITORED BY P-32 POSTLABELING - RELATIONSHIPS TO CANCER AND AGING
RANDERATH, K
LI, DH
NATH, R
RANDERATH, E
EXPERIMENTAL GERONTOLOGY, 1992, 27 (5-6) : 533 - 549
[40] P-32-postlabelling/HPLC analysis of DNA adducts formed from chrysene and its metabolites
Pfau, W
Seidel, A
Phillips, DH
Oesch, F
Grover, PL
POLYCYCLIC AROMATIC COMPOUNDS, 1996, 10 (1-4) : 219 - 226

← 1 2 3 4 5 →