In vitro ovarian tumor growth and treatment response dynamics visualized with time-lapse OCT imaging

被引：31

作者：

Evans, Conor L. ^{[1
]}

Rizvi, Imran ^{[1
,2
]}

Hasan, Tayyaba ^{[1
]}

de Boer, Johannes F. ^{[3
]}

机构：

[1] Harvard Univ, Sch Med, Wellman Ctr Photomed, Massachusetts Gen Hosp, Boston, MA 02114 USA

[2] Dartmouth Coll, Thayer Sch Engn, Hanover, NH 03755 USA

[3] Vrije Univ, Dept Phys & Astron, NL-1081 HV Amsterdam, Netherlands

来源：

OPTICS EXPRESS | 2009年 / 17卷 / 11期

基金：

美国国家卫生研究院;

关键词：

OPTICAL COHERENCE TOMOGRAPHY; CANCER; VIVO; MICROSCOPY; APOPTOSIS; MATRIX; TISSUE; CELLS; MODEL;

D O I：

10.1364/OE.17.008892

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

In vitro three-dimensional models for metastatic ovarian cancer have been useful for recapitulating the human disease. These spheroidal tumor cultures, however, can grow in excess of 1 mm in diameter, which are difficult to visualize without suitable imaging technology. Optical coherence tomography (OCT) is an ideal live imaging method for non-perturbatively visualizing these complex systems. OCT enabled detailed observations of the model at both nodular and cellular levels, revealing growth dynamics not previously observed. The development of a time-lapse OCT system, capable of automated, multidimensional acquisition, further provided insights into the growth and chemotherapeutic response of ovarian cancer. (c) 2009 Optical Society of America

引用

页码：8892 / 8906

页数：15

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