Enrichment Broth Improved Detection of Extended-Spectrum-Beta-Lactamase-Producing Bacteria in Throat and Rectal Surveillance Cultures of Samples from Patients in Intensive Care Units

被引:45
作者
Murk, Jean-Luc A. N. [1 ]
Heddema, Edou R. [2 ]
Hess, Dorine L. J. [1 ]
Bogaards, Johannes A. [1 ]
Vandenbroucke-Grauls, Christina M. J. E. [1 ]
Debets-Ossenkopp, Yvette J. [1 ]
机构
[1] Vrije Univ Amsterdam, Med Ctr, NL-1081 HV Amsterdam, Netherlands
[2] Maaslandhosp, Sittard, Netherlands
关键词
D O I
10.1128/JCM.01406-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We evaluated the use of a trypticase soy broth (TSB) for improving detection of extended-spectrum-beta-lactamase-producing (ESBL(+)) bacteria. Preenrichment of throat and rectal swabs in TSB prior to inoculation on solid medium doubled the number of ESBL(+) bacteria detected in samples obtained from patients in our intensive care unit.
引用
收藏
页码:1885 / 1887
页数:3
相关论文
共 6 条
[1]   Effects of selective decontamination of digestive tract on mortality and acquisition of resistant bacteria in intensive care: a randomised controlled trial [J].
de Jonge, E ;
Schultz, MJ ;
Spanjaard, L ;
Bossuyt, PMM ;
Vroom, MB ;
Dankert, J ;
Kesecioglu, J .
LANCET, 2003, 362 (9389) :1011-1016
[2]  
Grandin S, 2009, Pathol Biol (Paris), V57, pe37, DOI 10.1016/j.patbio.2008.03.008
[3]   Detection of β-lactamase-mediated resistance [J].
Livermore, DM ;
Brown, DFJ .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 48 :59-64
[4]   Extended-spectrum β-lactamase-producing enterobacteriaceae:: an emerging public-health concern [J].
Pitout, Johann D. D. ;
Laupland, Kevin B. .
LANCET INFECTIOUS DISEASES, 2008, 8 (03) :159-166
[5]   Characterization of the extended-spectrum β-lactamase reference strain, Klebsiella pneumoniae K6 (ATCC 700603), which produces the novel enzyme SHV-18 [J].
Rasheed, JK ;
Anderson, GJ ;
Yigit, H ;
Queenan, AM ;
Doménech-Sánchez, A ;
Swenson, JM ;
Biddle, JW ;
Ferraro, MJ ;
Jacoby, GA ;
Tenover, FC .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (09) :2382-2388
[6]   Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant Staphylococcus aureus:: a prospective study comparing 32 methods [J].
Safdar, N ;
Narans, L ;
Gordon, B ;
Maki, DG .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (07) :3163-3166