Oxidative potential and in vitro toxicity of particles generated by pyrotechnic smokes in human small airway epithelial cells

被引：7
作者：
de Lagarde, Violaine Martin [1 ]
Rogez-Florent, Tiphaine [1 ]
Cazier, Fabrice [2 ]
Dewaele, Dorothee [2 ]
Cazier-Dennin, Francine [3 ]
Ollivier, Alexane [1 ]
Janona, Marion [1 ]
Achard, Sophie [4 ]
Andre, Veronique [1 ]
Monteil, Christelle [1 ]
Corbiere, Cecile [1 ,5 ]
机构：
[1] Normandie Univ UNIROUEN, UNICAEN, ABTE, 14000 Caen, F-76000 Rouen, France
[2] Univ Littoral Cote dOpale, Ctr Commun Mesures, CCM, Dunkerque, France
[3] Univ Littoral Cote dOpale, EA 4492 UCEIV Unite Chim Environm & Interact Vivan, SFR Condorcet FR CNRS 417, Dunkerque, France
[4] Univ Paris, Fac Pharm, Inserm UMR1153 CRESS,HERA Hlth Environm Risk Asses, Paris, France
[5] Normandie Univ, UNIROUEN, UNICAEN, ABTE,France UFR Sante, Batiment Rech,22 Blvd Gambetta, F-76000 Rouen, France
来源：
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2022年 / 239卷
关键词：
Pyrotechnic smokes; Particles; Physico-chemical characterization; Oxidative Potential; Small Airway Epithelial Primary Cells; Toxicity; AIRBORNE PARTICULATE MATTER; INFLAMMATORY RESPONSES; IRRITATION POTENCY; CIGARETTE-SMOKE; STRESS; PM2.5; GENOTOXICITY; BIODIESEL; EXPOSURE; MACROPHAGES;
D O I：
10.1016/j.ecoenv.2022.113637
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Pyrotechnic smokes are widely used in civilian and military applications. The major issue arise from the release of particles after smoke combustion but the health risks related to their exposure are poorly documented whereas toxicity of airborne particles on the respiratory target are very well known. Therefore, this study aimed to explore the in vitro toxicity of the particle fraction of different pyrotechnic smokes.Particles from a red signalling smoke (RSS), an hexachloroethane-based obscuring smoke (HC-OS) and an anti intrusion smoke (AIS) were collected from the cloud. RSS particles displayed the highest organic fraction (quinones and polycyclic aromatic hydrocarbons) of the three samples characterized. AIS particles contained K and cholesterol derivatives. HC-OS particles were mainly metallic with very high concentrations of Al, Fe and Ca. Intrinsic oxidative potential of smoke particles was measured with two assays. Depletions of DTT by RSS particles was greater than depletion obtained with AIS and HC-OS particles but depletion of acid ascorbic (AA) was only observed with HC-OS particles. In vitro toxicity was assessed by exposing human small airway epithelial cells (SAEC) to various concentrations of particles. After 24 h of exposure, cell viability was not affected but significant modifications of mRNA expression of antioxidant (SOD-1 and-2, catalase, HO-1, NQO-1) and inflammatory markers (IL-6, IL-8, TNF-alpha) were observed and were dependent on smoke type. Particles rich in metal, such as HC-OS, induced a greatest depletion of AA and a greatest inflammatory response, whereas particles rich in organic compounds, such as RSS, induced a greatest DTT depletion and a greatest antioxidant response. In conclusion, the three smoke particles have an intrinsic oxidative potential and triggered a cell adaptive response.Our study improved the knowledge of particle toxicity of pyrotechnic smokes and scientific approach developed here could be used to study other type of particles.
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页数：11
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