POU/TBP cooperativity: A mechanism for enhancer action from a distance

被引:46
作者
Bertolino, E [1 ]
Singh, H [1 ]
机构
[1] Univ Chicago, Howard Hughes Med Inst, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA
关键词
D O I
10.1016/S1097-2765(02)00597-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Enhancers when functioning at a distance cannot effectively stimulate transcription from core promoters. We demonstrate that this is due to the inability of enhancer-bound activators to recruit TBP to a distal TATA box. Surprisingly, binding of a transcriptionally inert Oct-1 POU domain near a core promoter enables an enhancer to function from a distance. POU activity neither requires the coactivator OCA-B nor the interaction of TBP with TFIIA. Instead, the POU domain directly facilitates TBP recruitment to the promoter utilizing a bipartite interaction surface. These results establish that an interaction between the DNA binding domain of an activator and TBP can be used to stimulate transcription. Furthermore, they suggest a mechanism for long-range enhancer function in which a TBP complex is preassembled on a promoter via localized recruitment and then acted upon by distal activators.
引用
收藏
页码:397 / 407
页数:11
相关论文
共 50 条
[1]   Ordered recruitment of chromatin modifying and general transcription factors to the IFN-β promoter [J].
Agalioti, T ;
Lomvardas, S ;
Parekh, B ;
Yie, JM ;
Maniatis, T ;
Thanos, D .
CELL, 2000, 103 (04) :667-678
[2]   THE BASIC HELIX-LOOP-HELIX-ZIPPER DOMAIN OF TFE3 MEDIATES ENHANCER-PROMOTER INTERACTION [J].
ARTANDI, SE ;
COOPER, C ;
SHRIVASTAVA, A ;
CALAME, K .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (12) :7704-7716
[3]   2 REGULATORY ELEMENTS FOR IMMUNOGLOBULIN-KAPPA LIGHT CHAIN GENE-EXPRESSION [J].
BERGMAN, Y ;
RICE, D ;
GROSSCHEDL, R ;
BALTIMORE, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (22) :7041-7045
[4]   A novel homeobox protein which recognizes a TGT core and functionally interferes with a retinoid-responsive motif [J].
Bertolino, E ;
Reimund, B ;
WildtPerinic, D ;
Clerc, RG .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (52) :31178-31188
[5]  
BERTOLINO E, 2000, TRANSCRIPTION FACTOR, P294
[6]   c-Myc target gene specificity is determined by a post-DNA-binding mechanism [J].
Boyd, KE ;
Wells, J ;
Gutman, J ;
Bartley, SM ;
Farnham, PJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (23) :13887-13892
[7]   TRANSCRIPTIONAL SILENCING IN YEAST IS ASSOCIATED WITH REDUCED NUCLEOSOME ACETYLATION [J].
BRAUNSTEIN, M ;
ROSE, AB ;
HOLMES, SG ;
ALLIS, CD ;
BROACH, JR .
GENES & DEVELOPMENT, 1993, 7 (04) :592-604
[8]   Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo [J].
Bryant, GO ;
Martel, LS ;
Burley, SK ;
Berk, AJ .
GENES & DEVELOPMENT, 1996, 10 (19) :2491-2504
[9]   Cloning and biochemical characterization of TAF-172, a human homolog of yeast Mot1 [J].
Chicca, JJ ;
Auble, DT ;
Pugh, BF .
MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (03) :1701-1710
[10]   Association of human TFIID-promoter complexes with silenced mitotic chromatin in vivo [J].
Christova, R ;
Oelgeschläger, T .
NATURE CELL BIOLOGY, 2002, 4 (01) :79-82