The apoptosis linked gene ALG-2 is dysregulated in tumors of various origin and contributes to cancer cell viability

被引：42

作者：

la Cour, Jonas M. ^{[1
]}

Hoj, Berit R. ^{[1
]}

Mollerup, Jens ^{[1
]}

Simon, Ronald ^{[2
]}

Sauter, Guido ^{[2
]}

Berchtold, Martin W. ^{[1
]}

机构：

[1] Univ Copenhagen, Dept Mol Biol, Copenhagen Bioctr, DK-2200 Copenhagen N, Denmark

[2] Univ Med Ctr Hamburg Eppendorf, Dept Pathol, Hamburg, Germany

来源：

MOLECULAR ONCOLOGY | 2008年 / 1卷 / 04期

关键词：

Calcium binding proteins; Tissue microarray; Gene silencing; ALG-2;

D O I：

10.1016/j.molonc.2007.08.002

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The apoptosis linked gene-2 (ALG-2), discovered as a proapoptotic calcium binding protein, has recently been found upregulated in lung cancer tissue indicating that this protein may play a role in the pathology of cancer cells and/or may be a tumor marker. Using immunohistochemistry on tissue microarrays we analysed the expression of ALG-2 in 7371 tumor tissue samples of various origin as well as in 749 normal tissue samples. Most notably, ALG-2 was upregulated in mesenchymal tumors. No correlation was found between ALG-2 staining intensity and survival of patients with lung, breast or colon cancer. siRNA mediated ALG-2 downregulation led to a significant reduction in viability of HeLa cells indicating that ALG-2 may contribute to tumor development and expansion. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：431 / 439

页数：9

共 31 条

[1] Alix (ALG-2-interacting protein X), a protein involved in apoptosis, binds to endophilins and induces cytoplasmic vacuolization
Chatellard-Causse, C
Blot, B
Cristina, N
Torch, S
Missotten, M
Sadoul, R
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (32) : 29108 - 29115
[2] Apoptosis-linked gene-2 connects the Raf-1 and ASK1 signalings
Chen, CM
Sytkowski, AJ
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 333 (01) : 51 - 57
[3] The calcium binding protein ALG-2 binds and stabilizes Scotin, a p53-inducible gene product localized at the endoplasmic reticulum membrane
Draeby, Ingrid
Woods, Yvonne L.
la Cour, Jonas M.
Mollerup, Jens
Bourdon, Jean-Christophe
Berchtold, Martin W.
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2007, 467 (01) : 87 - 94
[4] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, SM
Harborth, J
Lendeckel, W
Yalcin, A
Weber, K
Tuschl, T
[J]. NATURE, 2001, 411 (6836) : 494 - 498
[5] Apoptosis-linked gene 2-deficient mice exhibit normal T-cell development and function
Jang, IK
Hu, RJ
Lacaná, E
D'Adamio, L
Gu, H
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2002, 22 (12) : 4094 - 4100
[6] The penta-EF-hand protein ALG-2 interacts directly with the ESCRT-I component TSG101, and Ca2+-dependently co-localizes to aberrant endosomes with dominant-negative AAA ATPase SKD1/Vps4B
Katoh, K
Suzuki, H
Terasawa, Y
Mizuno, T
Yasuda, J
Shibata, H
Maki, M
[J]. BIOCHEMICAL JOURNAL, 2005, 391 : 677 - 685
[7] Tissue microarrays for high-throughput molecular profiling of tumor specimens
Kononen, J
Bubendorf, L
Kallioniemi, A
Bärlund, M
Schraml, P
Leighton, S
Torhorst, J
Mihatsch, MJ
Sauter, G
Kallioniemi, OP
[J]. NATURE MEDICINE, 1998, 4 (07) : 844 - 847
[8] Targeted deletion of the Tsg101 gene results in cell cycle arrest at G1/S and p53-independent cell death
Krempler, A
Henry, MD
Triplett, AA
Wagner, KU
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (45) : 43216 - 43223
[9] Up-regulation of ALG-2 cancer tissue in hepatomas and lung
la Cour, JM
Mollerup, J
Winding, P
Tarabykina, S
Sehested, M
Berchtold, MW
[J]. AMERICAN JOURNAL OF PATHOLOGY, 2003, 163 (01) : 81 - 89
[10] ALG-2 oscillates in subcellular localization, unitemporally with calcium oscillations
la Cour, Jonas M.
Mollerup, Jens
Berchtold, Martin Werner
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 353 (04) : 1063 - 1067

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