Sol-gel derived nano-structured zinc oxide film for sexually transmitted disease sensor

被引：52

作者：

Ansari, Anees A. ^{[1
]}

Singh, Renu ^{[1
]}

Sumana, G. ^{[1
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

来源：

ANALYST | 2009年 / 134卷 / 05期

关键词：

ELECTROCHEMICAL DETECTION; METHYLENE-BLUE; COMPOSITE FILM; DNA DETECTION; HYBRIDIZATION; BIOSENSOR; GLUCOSE;

D O I：

10.1039/b817562d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A 20-mer thiolated oligonucleotide probe (th-ssDNA) specific to Neisseria gonorrhoeae immobilized onto a sol-gel derived nano-structured zinc oxide (ZnO) film dip-coated onto an indium-tin-oxide (ITO) glass substrate has been used for the fabrication of a DNA biosensor for sexually transmitted disease (gonorrhoea) detection using hybridization technique. The results of characterization studies carried out on this th-ssDNA-ZnO/ITO bioelectrode using X-ray diffraction, UV-Visible, Fourier-transform infrared, scanning electron microscopy and electrochemical techniques reveal the linearity as 0.000524 fmol-0.524 nmol, with a detection limit of 0.000704 fmol within 60 s.

引用

页码：997 / 1002

页数：6

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