Ergosterol determination in Saccharomyces cerevisiae. Comparison of different methods

被引：24
作者：
Lamacka, M
Sajbidor, J
机构：
[1] Department of Biochemical Technology, Chemical Faculty, Slovak Technical University, Radlinského 9
来源：
BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 10期
关键词：
D O I：
10.1023/A:1018484015806
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Several methods for ergosterol determination in two different Saccharomyces cerevisiae strains were tested. The best results were obtained by a simple method including saponification with 8 M KOH, extraction of non-saponifiable lipids and ergosterol determination by reversed phase HPLC. The methods in which the samples were treated with 0.1 M HCl prior to hydrolysis with 2.5 or 4.5 M water-ethanolic KOH underestimated the ergosterol contents.
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收藏
页码：723 / 725
页数：3
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