Aptamer-based assay of biomolecules: Recent advances in electro-analytical approach

被引：92

作者：

Hasanzadeh, Mohammad ^{[1
,2
]}

Shadjou, Nasrin ^{[3
,4
]}

de la Guardia, Miguel ^{[5
]}

机构：

[1] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz 51664, Iran

[2] Tabriz Univ Med Sci, Pharmaceut Anal Res Ctr, Tabriz 51664, Iran

[3] Urmia Univ, Nano Technol Res Ctr, Dept Neurochem, Orumiyeh, Iran

[4] Urmia Univ, Fac Sci, Dept Nanochem, Orumiyeh, Iran

[5] Univ Valencia, Dept Analyt Chem, Dr Moliner 50, E-46100 Valencia, Spain

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2017年 / 89卷

关键词：

Aptamer; Electroanalysis; Biosensor; Protein; Biomolecules; ROLLING CIRCLE AMPLIFICATION; ELECTROCHEMICAL APTASENSOR; SIGNAL AMPLIFICATION; LABEL-FREE; RNA APTAMER; PLASMINOGEN-ACTIVATOR; SENSITIVE DETECTION; RECOGNITION SYSTEM; DOPAMINE RELEASE; IMMUNOGLOBULIN-E;

D O I：

10.1016/j.trac.2017.02.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electrochemical aptasensors, which are based on the specificity of aptamer-target recognition, with electrochemical transduction for analytical purposes have received particular attention due to their high sensitivity and selectivity, simple instrumentation, as well as low production cost. This review summarizes advances from 2009 to January 2017 in the development of electrochemical aptasensors for biological molecules, including small molecules, nucleic acids, and proteins. Various aptasensing strategies are assessed according to their potential for reaching relevant limits of sensitivity, specificity, and degrees of multiplexing. Furthermore, we address the remaining challenges and opportunities to integrate electrochemical aptasensing platforms into point-of-care solutions. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：119 / 132

页数：14

共 71 条

[1] Surface state of the dopamine RNA aptamer affects specific recognition and binding of dopamine by the aptamer-modified electrodes [J].