Neuro-optical microfluidic platform to study injury and regeneration of single axons

被引：64

作者：

Kim, Young-tae ^{[1
]}

Karthikeyan, Kailash ^{[1
]}

Chirvi, Sajal ^{[1
]}

Dave, Digant P. ^{[1
]}

机构：

[1] Univ Texas Arlington, Dept Bioengn, Arlington, TX 76019 USA

来源：

LAB ON A CHIP | 2009年 / 9卷 / 17期

关键词：

CULTURE PLATFORM; NERVE; GROWTH; POLY(DIMETHYLSILOXANE); NANOSURGERY; ANTAGONIST; EXPRESSION; PROCESSOR; NEURONS;

D O I：

10.1039/b903720a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a neuro-optical microfluidic platform for studying injury and subsequent regeneration of individual mammalian axons. This platform consists of three components integrated on an inverted microscope, which include a compartmentalized neuronal culture microfluidic device, a femtosecond laser to enable precise axotomy, and a custom built mini cell culture incubator for continuous long term observation of post injury events. We demonstrate the unique capabilities of the platform by injuring individual central and peripheral nervous system axons and monitoring the post injury sequence of events from initial degeneration to subsequent regeneration. This platform will enable study and understanding of neuronal response to injury that is currently not possible with conventional cell culture platform and tools.

引用

页码：2576 / 2581

页数：6

共 2 条

[1] Microfluidic devices as model platforms of CNS injury-ischemia to study axonal regeneration by regulating mitochondrial transport and bioenergetic metabolism
Huang, Ning
Sheng, Zu-Hang
CELL REGENERATION, 2022, 11 (01)
[2] In-vitro study of monocytic THP-1 leukemia cell membrane elasticity with a single-cell microfluidic-assisted optical trapping system
Ombid, Ric John L.
Oyong, Glenn G.
Cabrera, Esperanza C.
Espulgar, Wilfred, V
Saito, Masato
Tamiya, Eiichi
Pobre, Romeric F.
BIOMEDICAL OPTICS EXPRESS, 2020, 11 (10) : 6027 - 6037

← 1 →