Physico-chemical characterization of dairy gel obtained by a proteolytic extract from Calotropis procera - A comparison with chymosin

被引：6

作者：

Rayanatou, Issa Ado ^{[1
,2
,3
]}

Mahamadou, ElHadji Gounga ^{[3
]}

Garric, Gilles ^{[1
,2
]}

Harel-Oger, Marielle ^{[1
,2
]}

Leduc, Arlette ^{[1
,2
]}

Jardin, Julien ^{[1
,2
]}

Briard-Bion, Valerie ^{[1
,2
]}

Cauty, Chantal ^{[1
,2
]}

Adakal, Hassane ^{[3
]}

Grongnet, Jean Francois ^{[2
]}

Gaucheron, Frederic ^{[1
,2
]}

机构：

[1] INRA, Agrocampus Quest, STLO, UMR 1253, F-35000 Rennes, France

[2] Agrocampus Quest, 65 Rue St Brieuc, F-35042 Rennes, France

[3] Univ Dandicko Dankoulodo Maradi, Fac Agron & Sci Environm, BP 465, Ads Maradi, Niger

来源：

FOOD CHEMISTRY | 2017年 / 232卷

关键词：

Cheese; Peptides; Whey; Curd; Coagulation; Calotropis; Chymosin; Dairy gel; CYSTEINE PROTEASE; CHEESE-MAKING; LATEX; MILK; PURIFICATION; PEPTIDASES; INSIGHTS; PH;

D O I：

10.1016/j.foodchem.2017.04.039

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Chymosin is the major enzyme used in cheesemaking but latex enzymes are also used. The aim of this work was to characterize the composition and the structure of dairy gel obtained by an extract of Calotropis procera leaves in comparison with those obtained by chymosin. The biochemical and mineral compositions of the curds and the cheese yields obtained by using Calotropis procera extract or chymosin were relatively similar. Quantitative and qualitative evaluations of proteolysis after milk coagulation, determined by the non-protein nitrogen content and chromatography coupled to mass spectrometry, indicated that Calotropis procera extract was more proteolytic than chymosin and that j-casein was proteolyzed. The main consequence of proteolysis by Calotropis procera extract or chymosin was the formation of a similar and regular network with the presence of aggregates of casein micelles. These results support that Calotropis procera extract can be used as effective coagulant in cheesemaking. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：405 / 412

页数：8

共 37 条

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