Tissue factor expression in mesothelial cells: Induction both in vivo and in vitro

被引:20
作者
Bottles, KD
Laszik, Z
Morrissey, JH
Kinasewitz, GT
机构
[1] UNIV OKLAHOMA,HLTH SCI CTR,DEPT PATHOL,OKLAHOMA CITY,OK 73190
[2] OKLAHOMA MED RES FDN,CARDIOVASC BIOL RES PROGRAM,OKLAHOMA CITY,OK 73104
关键词
D O I
10.1165/ajrcmb.17.2.2438
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Exudative pleural effusions are characterized by a high protein content and frequently progress to loculation and fibrosis. To test the hypothesis that tissue factor (TF) plays an integral role in this process, we in vestigated the expression of TF by human mesothelial cells (HMC) both in vivo and bl vitro, and measured the effect of serum on HMC expression of TF in vitro, In vitro TF expression was not detected in HMC of normal pleura, but was detected in HMC of pleura overlying inflamed lung. In vitro, quiescent HMC demonstrated negligible levels of: TF expression; however, upon serum stimulation there was a marked induction in both TF protein level and activity, peaking at 8-9 h. In contrast, treating quiescent HMC with plasma resulted in a further small, but significant, decrease in TF expression. This serum-induced rise in TF was also reflected in TF mRNA levels and did not require de novo protein synthesis, These results suggest that induction of HMC TF expression may be important in triggering both the intrapleural activation of prothrombin and the deposition of fibrin characteristic of inflammatory effusions.
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收藏
页码:164 / 172
页数:9
相关论文
共 40 条
[1]   PLEURAL CELL BIOLOGY IN HEALTH AND DISEASE [J].
ANTONY, VB ;
SAHN, SA ;
MOSSMAN, B ;
GAIL, DB ;
KALICA, A .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1992, 145 (05) :1236-1239
[2]   HETEROGENEOUS REGULATION OF CONSTITUTIVE THROMBOMODULIN OR INDUCIBLE TISSUE-FACTOR ACTIVITIES ON THE SURFACE OF HUMAN SAPHENOUS-VEIN ENDOTHELIAL-CELLS IN CULTURE FOLLOWING STIMULATION BY INTERLEUKIN-1, TUMOR-NECROSIS-FACTOR, THROMBIN OR PHORBOL ESTER [J].
ARCHIPOFF, G ;
BERETZ, A ;
FREYSSINET, JM ;
KLEINSOYER, C ;
BRISSON, C ;
CAZENAVE, JP .
BIOCHEMICAL JOURNAL, 1991, 273 :679-684
[3]  
Bignon J., 1985, PLEURA HLTH DISEASE, P417
[4]   SERUM STIMULATION OF QUIESCENT HUMAN-FIBROBLASTS INDUCES THE SYNTHESIS OF TISSUE FACTOR MESSENGER-RNA FOLLOWED BY THE APPEARANCE OF TISSUE FACTOR ANTIGEN AND PROCOAGULANT ACTIVITY [J].
BLOEM, LJ ;
CHEN, L ;
KONIGSBERG, WH ;
BACH, R .
JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 139 (02) :418-423
[5]   PATHOPHYSIOLOGY OF THE PLEURAL SPACE [J].
BOGGS, DS ;
KINASEWITZ, GT .
AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 1995, 309 (01) :53-59
[6]   DEXAMETHASONE ENHANCES AGONIST INDUCTION OF TISSUE FACTOR IN MONOCYTES BUT NOT IN ENDOTHELIAL-CELLS [J].
BOTTLES, KD ;
MORRISSEY, JH .
BLOOD COAGULATION & FIBRINOLYSIS, 1993, 4 (03) :405-414
[7]  
CONKLING PR, 1988, BLOOD, V72, P128
[8]   FUNCTIONAL TISSUE FACTOR IS ENTIRELY CELL-SURFACE EXPRESSED ON LIPOPOLYSACCHARIDE-STIMULATED HUMAN-BLOOD MONOCYTES AND A CONSTITUTIVELY TISSUE FACTOR PRODUCING NEOPLASTIC CELL-LINE [J].
DRAKE, TA ;
RUF, W ;
MORRISSEY, JH ;
EDGINGTON, TS .
JOURNAL OF CELL BIOLOGY, 1989, 109 (01) :389-395
[9]  
DRAKE TA, 1989, AM J PATHOL, V134, P1087
[10]   REGULATION OF EXTRAVASCULAR COAGULATION BY MICROVASCULAR PERMEABILITY [J].
DVORAK, HF ;
SENGER, DR ;
DVORAK, AM ;
HARVEY, VS ;
MCDONAGH, J .
SCIENCE, 1985, 227 (4690) :1059-1061