Heterochromatin protein 1 is recruited to various types of DNA damage

被引：212

作者：

Luijsterburg, Martijn S. ^{[1
,7
]}

Dinant, Christoffel ^{[3
,4
,8
]}

Lans, Hannes ^{[3
]}

Stap, Jan ^{[2
]}

Wiernasz, Elzbieta ^{[1
,5
]}

Lagerwerf, Saskia ^{[6
]}

Warmerdam, Daniel O. ^{[3
]}

Lindh, Michael ^{[7
]}

Brink, Maartje C. ^{[1
]}

Dobrucki, Jurek W. ^{[5
]}

Aten, Jacob A. ^{[2
]}

Fousteri, Maria I. ^{[6
]}

Jansen, Gert ^{[3
]}

Dantuma, Nico P. ^{[7
]}

Vermeulen, Wim ^{[3
]}

Mullenders, Leon H. F. ^{[6
]}

Houtsmuller, Adriaan B. ^{[4
]}

Verschure, Pernette J. ^{[1
]}

van Driel, Roel ^{[1
]}

机构：

[1] Univ Amsterdam, Acad Med Ctr, Swammerdam Inst Life Sci, NL-1012 WX Amsterdam, Netherlands

[2] Univ Amsterdam, Acad Med Ctr, Dept Cell Biol & Histol, Ctr Microscop Res, NL-1012 WX Amsterdam, Netherlands

[3] Erasmus MC, Dept Cell Biol & Genet, NL-3000 CA Rotterdam, Netherlands

[4] Erasmus MC, Josephine Nefkens Inst, Dept Pathol, NL-3000 CA Rotterdam, Netherlands

[5] Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Div Cell Biophys, PL-30387 Krakow, Poland

[6] Leiden Univ, Med Ctr, Dept Toxicogenet, NL-2300 RC Leiden, Netherlands

[7] Karolinska Inst, Dept Cell & Mol Biol, S-17177 Stockholm, Sweden

[8] Danish Canc Soc, Inst Canc Biol, Ctr Genotox Stress Res, DK-2100 Copenhagen, Denmark

来源：

JOURNAL OF CELL BIOLOGY | 2009年 / 185卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

DOUBLE-STRAND BREAKS; PERICENTRIC HETEROCHROMATIN; MAMMALIAN-CELLS; REPAIR FACTORS; HP1; PROTEINS; DYNAMICS; TRANSCRIPTION; SITES; MAINTENANCE; INDUCTION;

D O I：

10.1083/jcb.200810035

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Heterochromatin protein 1 (HP1) family members are chromatin-associated proteins involved in transcription, replication, and chromatin organization. We show that HP1 isoforms HP1-alpha, HP1-beta, and HP1-gamma are recruited to ultraviolet (UV)-induced DNA damage and double-strand breaks (DSBs) in human cells. This response to DNA damage requires the chromo shadow domain of HP1 and is independent of H3K9 trimethylation and proteins that detect UV damage and DSBs. Loss of HP1 results in high sensitivity to UV light and ionizing radiation in the nematode Caenorhabditis elegans, indicating that HP1 proteins are essential components of DNA damage response (DDR) systems. Analysis of single and double HP1 mutants in nematodes suggests that HP1 homologues have both unique and overlapping functions in the DDR. Our results show that HP1 proteins are important for DNA repair and may function to reorganize chromatin in response to damage.

引用

页码：577 / 586

页数：10

共 50 条

[1]

AGARWAL BK, 1981, J PHYS F MET PHYS, V11, P1303, DOI 10.1088/0305-4608/11/6/014

[2] Dynamics of DNA double-strand breaks revealed by clustering of damaged chromosome domains [J].

Aten, JA ;

Stap, J ;

Krawczyk, PM ;

van Oven, CH ;

Hoebe, RA ;

Essers, J ;

Kanaar, R .

SCIENCE, 2004, 303 (5654) :92-95

[3] HP1-β is required for development of the cerebral neocortex and neuromuscular junctions [J].

Aucott, Rebecca ;

Bullwinkel, Joern ;

Yu, Yang ;

Shi, Wei ;

Billur, Mustafa ;

Brown, Jeremy P. ;

Menzel, Ursula ;

Kioussis, Dimitris ;

Wang, Guozheng ;

Reisert, Ingrid ;

Weimer, Joerg ;

Pandita, Raj K. ;

Sharma, Girdhar G. ;

Pandita, Tej K. ;

Fundele, Reinald ;

Singh, Prim B. .

JOURNAL OF CELL BIOLOGY, 2008, 183 (04) :597-606

[4] HP1-β mobilization promotes chromatin changes that initiate the DNA damage response [J].

Ayoub, Nabieh ;

Jeyasekharan, Anand D. ;

Bernal, Juan A. ;

Venkitaraman, Ashok R. .

NATURE, 2008, 453 (7195) :682-U14

[5] DNA damage checkpoints: from initiation to recovery or adaptation [J].

Bartek, Jiri ;

Lukas, Jiri .

CURRENT OPINION IN CELL BIOLOGY, 2007, 19 (02) :238-245

[6] DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A [J].

Bergink, Steven ;

Salomons, Florian A. ;

Hoogstraten, Deborah ;

Groothuis, Tom A. M. ;

de Waard, Harm ;

Wu, Junxin ;

Yuan, Li ;

Citterio, Elisabetta ;

Houtsmuller, Adriaan B. ;

Neefjes, Jacques ;

Hoeijmakers, Ian H. J. ;

Vermeulen, Wim ;

Dantuma, Nico P. .

GENES & DEVELOPMENT, 2006, 20 (10) :1343-1352

[7] Maintenance of stable heterochromatin domains by dynamic HP1 binding [J].

Cheutin, T ;

McNairn, AJ ;

Jenuwein, T ;

Gilbert, DM ;

Singh, PB ;

Misteli, T .

SCIENCE, 2003, 299 (5607) :721-725

[8] The C. elegans HP1 homologue HPL-2 and the LIN-13 zinc finger protein form a complex implicated in vulval development [J].

Coustham, Vincent ;

Bedet, Cecile ;

Monier, Karine ;

Schott, Sonia ;

Karali, Marianthi ;

Palladino, Francesca .

DEVELOPMENTAL BIOLOGY, 2006, 297 (02) :308-322

[9] Plasticity of HP1 proteins in mammalian cells [J].

Dialynas, George K. ;

Terjung, Stefan ;

Brown, Jeremy P. ;

Aucott, Rebecca L. ;

Baron-Luhr, Bettina ;

Singh, Prim B. ;

Georgatos, Spyros D. .

JOURNAL OF CELL SCIENCE, 2007, 120 (19) :3415-3424

[10] Activation of multiple DNA repair pathways by subnuclear damage induction methods [J].

Dinant, Christoffel ;

de Jager, Martijn ;

Essers, Jeroen ;

van Cappellen, Wiggert A. ;

Kanaar, Roland ;

Houtsmuller, Adriaan B. ;

Vermeulen, Wim .

JOURNAL OF CELL SCIENCE, 2007, 120 (15) :2731-2740

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