Downregulation of SCAI enhances glioma cell invasion and stem cell like phenotype by activating Wnt/β-catenin signaling

被引：37

作者：

Chen, Xiangrong ^{[1
]}

Hu, Weipeng ^{[1
]}

Xie, Baoyuan ^{[1
]}

Gao, Hongzhi ^{[1
]}

Xu, Chaoyang ^{[1
]}

Chen, Junyan ^{[1
]}

机构：

[1] Fujian Med Univ, Affiliated Hosp 2, Dept Neurosurg, Quanzhou 362000, Fujian Province, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 448卷 / 02期

关键词：

SCAI; Glioma; Wnt/beta-catenin; Invasion; Cancer stem cell; CANCER; PATHWAY; GLIOBLASTOMA;

D O I：

10.1016/j.bbrc.2014.04.098

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SCAI (suppressor of cancer cell invasion), has been recently characterized as a novel tumor suppressor that inhibits the invasive migration of several human tumor cells. However, the expression pattern, biological role and molecular mechanism of SCAI in human glioma remain unknown. In this study, we found that levels of SCAI protein and mRNA expression were significantly downregulated in glioma tissues and cell lines. Overexpression of SCAI inhibited, but silencing of SCAI robustly promoted the invasive and cancer stem cell-like phenotypes of glioma cells. Furthermore, we demonstrated that SCAI downregualtion activated the Wnt/beta-catenin signaling, and blockade of the Wnt/beta-catenin pathway abrogated the effects of SCAI downregulation on glioma cell aggressiveness. Taken together, our results provide the first demonstration of SCAI downregulation in glioma, and its downregulation contributes to increased glioma cell invasion and self-renewal by activating the Wnt/beta-catenin pathway. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：206 / 211

页数：6

共 20 条

[1] SOX9 is an intestine crypt transcription factor, is regulated by the Wnt pathway, and represses the CDX2 and MUC2 genes [J].