Biofunctionalization of Ulvan Scaffolds for Bone Tissue Engineering

被引：68

作者：

Dash, Mamoni ^{[1
]}

Samal, Sangram K. ^{[1
]}

Bartoli, Cristina ^{[2
]}

Morelli, Andrea ^{[2
]}

Smet, Philippe F. ^{[3
]}

Dubruel, Peter ^{[1
]}

Chiellini, Federica ^{[2
]}

机构：

[1] Univ Ghent, Polymer Chem & Biomat Res Grp, B-9000 Ghent, Belgium

[2] Univ Pisa, Univ Pisa UdR INSTM, Dept Chem & Ind Chem, I-1291 Pisa, Italy

[3] Univ Ghent, Dept Solid State Sci, Lumilab, B-9000 Ghent, Belgium

来源：

ACS APPLIED MATERIALS & INTERFACES | 2014年 / 6卷 / 05期

关键词：

ulvan; scaffolds; enzyme; mineral; tissue engineering; MINERALIZATION; HYDROXYAPATITE; HYDROGELS;

D O I：

10.1021/am404912c

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Photo-cross-linked ulvan scaffolds were designed with the aim to induce and support enzyme mediated formation of apatite minerals, in the absence of osteogenic growth factors. Scaffold formation with a desired geometry was investigated using chemically modified ulvan bearing radically polymerizable groups. Further bioactivity was incorporated by the use of alkaline phosphatase (ALP) induced minerals. Successful modification of UV cross-linked ulvan scaffolds was revealed by H-1 NMR The presence of the mineral formation was evidenced by Raman spectroscopy and XRD techniques. Investigations of the morphology Confirmed the homogeneous mineralization using ALP. The MC3T3 cell activity clearly showed that the mineralization of the biofunctionalized ulvan scaffolds was effective in improving the cellular activity.

引用

页码：3211 / 3218

页数：8

共 31 条

[11]

Gkioni K, 2010, TISSUE ENG PART B-RE, V16, P577, DOI [10.1089/ten.teb.2010.0462, 10.1089/ten.TEB.2010.0462]

[12] Cell encapsulation using biopolymer gels for regenerative medicine [J].

Hunt, Nicola C. ;

Grover, Liam M. .

BIOTECHNOLOGY LETTERS, 2010, 32 (06) :733-742

[13] Preparation and Characterization of Aloe Vera Blended Collagen-Chitosan Composite Scaffold for Tissue Engineering Applications [J].

Jithendra, Panneerselvam ;

Rajam, Abraham Merlin ;

Kalaivani, Thambiran ;

Mandal, Asit Baran ;

Rose, Chellan .

ACS APPLIED MATERIALS & INTERFACES, 2013, 5 (15) :7291-7298

[14] SIMPLE AND SENSITIVE METHOD FOR THE QUANTITATIVE ESTIMATION OF COLLAGEN [J].

JUNQUEIRA, LCU ;

BIGNOLAS, G ;

BRENTANI, RR .

ANALYTICAL BIOCHEMISTRY, 1979, 94 (01) :96-99

[15] Current trends and future perspectives of bone substitute materials - From space holders to innovative biomaterials [J].

Kolk, Andreas ;

Handschel, Joerg ;

Drescher, Wolf ;

Rothamel, Daniel ;

Kloss, Frank ;

Blessmann, Marco ;

Heiland, Max ;

Wolff, Klaus-Dietrich ;

Smeets, Ralf .

JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY, 2012, 40 (08) :706-718

[16] Review: Mineralization of synthetic polymer scaffolds for bone tissue engineering [J].

Kretlow, James D. ;

Mikos, Antonios G. .

TISSUE ENGINEERING, 2007, 13 (05) :927-938

[17] Synthetic bone graft substitutes [J].

Moore, WR ;

Graves, SE ;

Bain, GI .

ANZ JOURNAL OF SURGERY, 2001, 71 (06) :354-361

[18] Ulvan as a New Type of Biomaterial from Renewable Resources: Functionalization and Hydrogel Preparation [J].

Morelli, Andrea ;

Chiellini, Federica .

MACROMOLECULAR CHEMISTRY AND PHYSICS, 2010, 211 (07) :821-832

[19] Biodegradable polymers as biomaterials [J].

Nair, Lakshmi S. ;

Laurencin, Cato T. .

PROGRESS IN POLYMER SCIENCE, 2007, 32 (8-9) :762-798

[20] Bone structure and formation: A new perspective [J].

Olszta, Matthew J. ;

Cheng, Xingguo ;

Jee, Sang Soo ;

Kumar, Rajendra ;

Kim, Yi-Yeoun ;

Kaufman, Michael J. ;

Douglas, Elliot P. ;

Gower, Laurie B. .

MATERIALS SCIENCE & ENGINEERING R-REPORTS, 2007, 58 (3-5) :77-116

← 1 2 3 4 →