Biofunctionalization of Ulvan Scaffolds for Bone Tissue Engineering

被引：68
作者：
Dash, Mamoni [1 ]
Samal, Sangram K. [1 ]
Bartoli, Cristina [2 ]
Morelli, Andrea [2 ]
Smet, Philippe F. [3 ]
Dubruel, Peter [1 ]
Chiellini, Federica [2 ]
机构：
[1] Univ Ghent, Polymer Chem & Biomat Res Grp, B-9000 Ghent, Belgium
[2] Univ Pisa, Univ Pisa UdR INSTM, Dept Chem & Ind Chem, I-1291 Pisa, Italy
[3] Univ Ghent, Dept Solid State Sci, Lumilab, B-9000 Ghent, Belgium
来源：
ACS APPLIED MATERIALS & INTERFACES | 2014年 / 6卷 / 05期
关键词：
ulvan; scaffolds; enzyme; mineral; tissue engineering; MINERALIZATION; HYDROXYAPATITE; HYDROGELS;
D O I：
10.1021/am404912c
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Photo-cross-linked ulvan scaffolds were designed with the aim to induce and support enzyme mediated formation of apatite minerals, in the absence of osteogenic growth factors. Scaffold formation with a desired geometry was investigated using chemically modified ulvan bearing radically polymerizable groups. Further bioactivity was incorporated by the use of alkaline phosphatase (ALP) induced minerals. Successful modification of UV cross-linked ulvan scaffolds was revealed by H-1 NMR The presence of the mineral formation was evidenced by Raman spectroscopy and XRD techniques. Investigations of the morphology Confirmed the homogeneous mineralization using ALP. The MC3T3 cell activity clearly showed that the mineralization of the biofunctionalized ulvan scaffolds was effective in improving the cellular activity.
引用
收藏
页码：3211 / 3218
页数：8
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