Flagellin and F4 fimbriae have opposite effects on biofilm formation and quorum sensing in F4ac+enterotoxigenic Escherichia coli

被引：39
作者：
Zhou, Mingxu [1 ,2 ]
Guo, Zhiyan [1 ,2 ]
Yang, Yang [1 ,2 ]
Duan, Qiangde [1 ,3 ]
Zhang, Qi [1 ,2 ]
Yao, Fenghua [1 ,2 ]
Zhu, Jun [1 ,2 ]
Zhang, Xinjun [1 ,2 ]
Hardwidge, Philip R. [4 ]
Zhu, Guoqiang [1 ,2 ]
机构：
[1] Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China
[2] Jiangsu Co Innovat Ctr Important Anim Infect Dis, Yangzhou 225009, Peoples R China
[3] Weinan Vocat & Tech Coll, Coll Agr, Weinan 714000, Peoples R China
[4] Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA
来源：
VETERINARY MICROBIOLOGY | 2014年 / 168卷 / 01期
基金：
美国国家科学基金会;
关键词：
Flagellin; F4; fimbriae; Biofilm formation; Quorum sensing; IN-VITRO; SALMONELLA-TYPHIMURIUM; EXPRESSION; VIRULENCE; LUXS; MOTILITY; GENES; CONTRIBUTES; INFECTION; CELLS;
D O I：
10.1016/j.vetmic.2013.10.014
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Bacteria that form biofilms are often highly resistant to antibiotics and are capable of evading the host immune system. To evaluate the role of flagellin and F4 fimbriae on biofilm formation by enterotoxigenic Escherichia coli (ETEC), we deleted the fliC (encoding the major flagellin protein) and/or the faeG (encoding the major subunit of F4 fimbriae) genes from ETEC C83902. Biofilm formation was reduced in the fliC mutant but increased in the faeG mutant, as compared with the wild-type strain. The expression of AI-2 quorum sensing associated genes was regulated in the fliC and faeG mutants, consistent with the biofilm formation of these strains. But, deleting fliC and/or faeG also inhibited AI-2 quorum sensing activity. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：148 / 153
页数：6
相关论文
共 50 条
  • [41] Oral immunization against enterotoxigenic colibacillosis in weaned piglets by non-pathogenic Escherichia coli strain with K88 (F4) colonizing factors
    Alexa, P
    Hamrik, J
    Stouracova, K
    Salajka, E
    VETERINARNI MEDICINA, 2005, 50 (07) : 315 - 320
  • [42] New perspectives to the enterotoxigenic E. coli F4 porcine infection model: Susceptibility genotypes in relation to performance, diarrhoea and bacterial shedding
    Roubos-van den Hil, Petra J.
    Litjens, Ralph
    Oudshoorn, Anna-Katharina
    Resink, Jan Willem
    Smits, Coen H. M.
    VETERINARY MICROBIOLOGY, 2017, 202 : 58 - 63
  • [43] Refined mapping of the Escherichia coli F4ab/F4ac receptor gene(s) on pig chromosome 13
    Niu, X.
    Li, Y.
    Ding, X.
    Zhang, Q.
    ANIMAL GENETICS, 2011, 42 (05) : 552 - 555
  • [44] Escherichia coli F4 fimbriae specific llama single-domain antibody fragments effectively inhibit bacterial adhesion in vitro but poorly protect against diarrhoea
    Harmsen, MM
    van Solt, CB
    Hoogendoorn, A
    van Zijderveld, FG
    Niewold, TA
    van der Meulen, J
    VETERINARY MICROBIOLOGY, 2005, 111 (1-2) : 89 - 98
  • [45] Maillard neoglycans as inhibitors for in vitro adhesion of F4+ enterotoxigenic Escherichia coli to piglet intestinal cells
    Manuel Sarabia-Sainz, Hector
    Mata Haro, Veronica
    Sarabia Sainz, Jose Andre-i
    Vazquez-Moreno, Luz
    Ramos-Clamont Montfort, Gabriela
    ACTA BIOCHIMICA POLONICA, 2017, 64 (04) : 679 - 686
  • [46] The immune response of pregnant sow after vaccination with crude fimbriae (F4) extracts vaccine and immunoprotection of nursery pig against pathogenic E. coli (F4+ ETEC)
    Nguyet, Luong Thi Yen
    Ounjai, Puey
    Ngamwongsatit, Natharin
    Kaeoket, Kampon
    ACTA TROPICA, 2024, 254
  • [47] Refined candidate region specified by haplotype sharing for Escherichia coli F4ab/F4ac susceptibility alleles in pigs
    Jacobsen, M.
    Kracht, S. S.
    Esteso, G.
    Cirera, S.
    Edfors, I.
    Archibald, A. L.
    Bendixen, C.
    Andersson, L.
    Fredholm, M.
    Jorgensen, C. B.
    ANIMAL GENETICS, 2010, 41 (01) : 21 - 25
  • [48] F1C Fimbriae Play an Important Role in Biofilm Formation and Intestinal Colonization by the Escherichia coli Commensal Strain Nissle 1917
    Lasaro, Melissa A.
    Salinger, Nina
    Zhang, Jing
    Wang, Yantao
    Zhong, Zhengtao
    Goulian, Mark
    Zhu, Jun
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (01) : 246 - 251
  • [49] Avirulent K88 (F4)+ Escherichia coli strains constructed to express modified enterotoxins protect young piglets from challenge with a virulent enterotoxigenic Escherichia coli strain that expresses the same adhesion and enterotoxins
    Santiago-Mateo, Kristina
    Zhao, Mojun
    Lin, Jun
    Zhang, Weiping
    Francis, David H.
    VETERINARY MICROBIOLOGY, 2012, 159 (3-4) : 337 - 342
  • [50] Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned piglets
    Fiil, Berthe Katrine
    Thrane, Sandra Wingaard
    Pichler, Michael
    Kittila, Tiia
    Ledsgaard, Line
    Ahmadi, Shirin
    Hermansen, Grith Miriam Maigaard
    Jelsbak, Lars
    Lauridsen, Charlotte
    Brix, Susanne
    Laustsen, Andreas Hougaard
    ISCIENCE, 2022, 25 (04)
12345