Identification and characterization of cis-regulatory elements insulator and repressor' in PPARD gene

被引：5
作者：
Yadav, Dilip K. [1 ]
Shrestha, Smeeta [1 ,2 ]
Dadhwal, Gunjan [1 ,3 ]
Chandak, Giriraj R. [1 ]
机构：
[1] Ctr Cellular & Mol Biol, CSIR, Genom Res Complex Dis GRC Grp, Hyderabad 500007, Telangana, India
[2] Dayananda Sagar Univ, Sch Basic & Appl Sci, Bldg 7,Shavige Malleshwara Hills, Bangalore 560078, Karnataka, India
[3] Univ Montreal, Dept Biochim & Med Mol, Montreal, PQ H3T 1J4, Canada
来源：
EPIGENOMICS | 2018年 / 10卷 / 05期
关键词：
cis-regulatory elements; ENCODE; enhancer; insulator; peroxisome proliferator-activated receptor beta/delta; repressor; Type; 2; diabetes; PROLIFERATOR-ACTIVATED RECEPTORS; ENHANCER-BLOCKING ACTIVITY; TRANSCRIPTIONAL REGULATION; GENOME BROWSER; DELTA; CTCF; METHYLATION; METABOLISM; BOUNDARIES; PROTEIN;
D O I：
10.2217/epi-2017-0139
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Aim: Identification and functional characterization of cis-regulatory elements in human PPARD gene. Methods: We used various bioinformatic tools on the publicly available human genome and Encyclopedia of DNA Elements databases to explore potential cis-regulatory elements in PPARD gene region. Results: We predicted an insulator and an enhancer element in intron 2 of PPARD gene. Functional characterization using transient transfection, reporter assay and CTCF binding confirmed the insulator status. However, the predicted enhancer element showed repressor/silencer activity. Finally, we observed a potential interaction between these two cis-regulatory elements which is in agreement with 5C-Encyclopedia of DNA Elements data. Conclusion: We report two functionally validated cis-regulatory elements in PPARD gene which will aid in understanding its regulation and role in metabolic functions.
引用
收藏
页码：613 / 627
页数：15
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