A chromatin-associated protein is encoded in a genomic region highly conserved in the Plasmodium genus

被引：17

作者：

Birago, C

Pace, T

Barca, S

Picci, L

Ponzi, M

机构：

[1] IST SUPER SANITA, LAB BIOL CELLULARE, I-00161 ROME, ITALY

[2] IST SUPER SANITA, IMMUNOL LAB, I-00161 ROME, ITALY

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1996年 / 80卷 / 02期

关键词：

Plasmodium; chromatin; nucleosome assembly proteins;

D O I：

10.1016/0166-6851(96)02680-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A single copy gene, pbB7, encoding a putative 26 kDa acidic protein has been isolated from Plasmodium berghei and appears to be part of a genomic region well conserved within the Plasmodium genus. The deduced amino acid sequence exhibits significant blocks of similarity with nucleosome assembly proteins from yeast and man. The nuclear localization of the natural protein and its close association with chromatin during the entire erythrocytic cycle of the parasite have been demonstrated using specific monoclonal antibodies against the pbB7 product expressed in Escherichia coli. These results suggest an involvement of this nuclear factor in the dynamics of chromatin packaging.

引用

收藏

页码：193 / 202

页数：10

相关论文

共 20 条

[11] TRANSCRIPTIONAL AND NUCLEOSOMAL CHARACTERIZATION OF A SUBTELOMERIC GENE-CLUSTER FLANKING A SITE OF CHROMOSOMAL REARRANGEMENTS IN PLASMODIUM-FALCIPARUM [J].

LANZER, M ;

DEBRUIN, D ;

WERTHEIMER, SP ;

RAVETCH, JV .

NUCLEIC ACIDS RESEARCH, 1994, 22 (20) :4176-4182

[12] TRANSCRIPTION MAPPING OF A 100 KB LOCUS OF PLASMODIUM-FALCIPARUM IDENTIFIES AN INTERGENIC REGION IN WHICH TRANSCRIPTION TERMINATES AND REINITIATES [J].

LANZER, M ;

DEBRUIN, D ;

RAVETCH, JV .

EMBO JOURNAL, 1992, 11 (05) :1949-1955

[13] CLONING AND SEQUENCING OF PLASMODIUM-FALCIPARUM DNA FRAGMENTS CONTAINING REPETITIVE REGIONS POTENTIALLY CODING FOR HISTIDINE-RICH PROTEINS - IDENTIFICATION OF 2 OVERLAPPING READING FRAMES [J].

LENSTRA, R ;

DAURIOL, L ;

ANDRIEU, B ;

LEBRAS, J ;

GALIBERT, F .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 146 (01) :368-377

[14] THE SEQUENCE OF PLASMODIUM-FALCIPARUM HISTONE H3 [J].

LONGHURST, HJ ;

HOLDER, AA .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1995, 69 (01) :111-113

[15] GENERATION OF CHROMOSOME SIZE POLYMORPHISM DURING INVIVO MITOTIC MULTIPLICATION OF PLASMODIUM-BERGHEI INVOLVES BOTH LOSS AND ADDITION OF SUBTELOMERIC REPEAT SEQUENCES [J].

PONZI, M ;

JANSE, CJ ;

DORE, E ;

SCOTTI, R ;

PACE, T ;

RETERINK, TJF ;

VANDERBERG, FM ;

MONS, B .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 41 (01) :73-82

[16] THE STRUCTURE OF THE CALMODULIN GENE OF PLASMODIUM-FALCIPARUM [J].

ROBSON, KJH ;

JENNINGS, MW .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 46 (01) :19-34

[17] MOLECULAR CHARACTERIZATION OF HNRP, A CDNA-ENCODING A HUMAN NUCLEOSOME-ASSEMBLY-PROTEIN-I-RELATED GENE-PRODUCT INVOLVED IN THE INDUCTION OF CELL-PROLIFERATION [J].

SIMON, HU ;

MILLS, GB ;

KOZLOWSKI, M ;

HOGG, D ;

BRANCH, D ;

ISHIMI, Y ;

SIMINOVITCH, KA .

BIOCHEMICAL JOURNAL, 1994, 297 :389-397

[18] PURIFICATION AND CHARACTERIZATION OF 2 PUTATIVE HLA CLASS-II ASSOCIATED PROTEINS - PHAPI AND PHAPII [J].

VAESEN, M ;

BARNIKOLWATANABE, S ;

GOTZ, H ;

AWNI, LA ;

COLE, T ;

ZIMMERMANN, B ;

KRATZIN, HD ;

HILSCHMANN, N .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1994, 375 (02) :113-126

[19] THE MOUSE TYPE-IV C-ABL GENE-PRODUCT IS A NUCLEAR-PROTEIN, AND ACTIVATION OF TRANSFORMING ABILITY IS ASSOCIATED WITH CYTOPLASMIC LOCALIZATION [J].

VANETTEN, RA ;

JACKSON, P ;

BALTIMORE, D .

CELL, 1989, 58 (04) :669-678

[20] CAN, A PUTATIVE ONCOGENE ASSOCIATED WITH MYELOID LEUKEMOGENESIS, MAY BE ACTIVATED BY FUSION OF ITS 3' 1/2 TO DIFFERENT GENES - CHARACTERIZATION OF THE SET GENE [J].

VONLINDERN, M ;

VANBAAL, S ;

WIEGANT, J ;

RAAP, A ;

HAGEMEIJER, A ;

GROSVELD, G .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (08) :3346-3355