Characterization of the lipid portion of pink shrimp commercial samples. The objective of this study was to describe the fresh cooled pink-shrimp (Penaeus brasiliensis and Penaeus paulensis) lipid. The total lipid content was 1,13+/-0,09 g/100g while fatty acid profile showed 32,9% saturated, 20,4% monounsaturated and 40,5% polyunsaturated. Eighteen fatty acids were detected, seven saturated (14:0, 15:0, 16:0, 17:0, 18:0, 20:0, 22:0), four monounsaturated (16:1w7, 17:1w9, 18:1w9, 20:1w9) and seven polyunsaturated (18:2w6, 18:3w3, 20:2w6, 20:4w6, 20:5w3, 22:5w3, 22:6w3). The free cholesterol content was 92 to 136 mg/100g with average of 118mg/100g. In same time, was observed the occurence of free 7-ketocholesterol, a product of cholesterol oxidation, in levels of 0,185 to 0,366 mug/g. The fatty acid profile was obtained by gas chromatography with a fused silica column Supelcowax 10. The cholesterol and 7-ketocholesterol was determined by high performance liquid chromatography using a mu-Porasil column, normal phase, and a diode array detector.