Molecular Imaging of β-Amyloid Plaques with Near-Infrared Boron Dipyrromethane (BODIPY)-Based Fluorescent Probes

被引：67

作者：

Watanabe, Hiroyuki ^{[1
]}

Ono, Masahiro ^{[1
]}

Matsumura, Kenji ^{[1
]}

Yoshimura, Masashi ^{[1
]}

Kimura, Hiroyuki ^{[1
]}

Saji, Hideo ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Pathofunct Bioanal, Kyoto 6068501, Japan

来源：

MOLECULAR IMAGING | 2013年 / 12卷 / 05期

基金：

日本学术振兴会;

关键词：

IN-VIVO DETECTION; ALZHEIMERS-DISEASE; PET; DERIVATIVES; BRAIN; F-19;

D O I：

10.2310/7290.2013.00049

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The formation of beta-amyloid (A beta) plaques is a critical neurodegenerative change in Alzheimer disease (AD). We designed and synthesized novel boron dipyrromethane (BODIPY)-based A beta probes (BAPs) and evaluated their utility for near-infrared fluorescence imaging of A beta plaques in the brain. In binding experiments in vitro, BAPs showed high affinity for synthetic A beta aggregates (K-d = 18-149 nM). Furthermore, BAPs clearly stained A beta plaques in sections of Tg2576 mice. In mouse brain tissue, BAPs showed sufficient uptake for optical imaging. In addition, ex vivo fluorescent staining of brain sections from Tg2576 mice after the injection of BAP-2 showed selective binding of A beta plaques with little nonspecific binding. BAPs may be useful as a near-infrared fluorescent probe for imaging A beta plaques.

引用

页码：338 / 347

页数：10

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